Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Theor Appl Genet. 2023 Aug 7;136(9):179. doi: 10.1007/s00122-023-04433-8.
Novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance were developed, and the resistance gene PmW6RS was physically mapped onto 6RS-0.58-0.66-bin corresponding to 18.38 Mb in Weining rye. Rye (Secale cereale L., RR) contains valuable genes for wheat improvement. However, most of the rye resistance genes have not been successfully used in wheat cultivars. Identification of new rye resistance genes and transfer of these genes to wheat by developing small fragment translocation lines will make these genes more usable for wheat breeding. In this study, a broad-spectrum powdery mildew resistance gene PmW6RS was localized on rye chromosome arm 6RS using a new set of wheat-rye disomic and telosomic addition lines. To further study and use PmW6RS, 164 wheat-rye 6RS translocation lines were developed by Coγ-ray irradiation. Seedling and adult stage powdery mildew resistance analysis showed that 106 of the translocation lines were resistant. A physical map of 6RS was constructed using the 6RS translocation and deletion lines, and PmW6RS was localized in the 6RS-0.58-0.66-bin, flanked by markers X6RS-3 and X6RS-10 corresponding to the physical interval of 50.23-68.61 Mb in Weining rye genome. A total of 23 resistance-related genes were annotated. Nine markers co-segregate with the 6RS-0.58-0.66-bin, which can be used to rapidly trace the 6RS fragment carrying PmW6RS. Small fragment translocation lines with powdery mildew resistance were backcrossed with wheat cultivars, and 39 agronomically acceptable homozygous 6RS small fragment translocation lines were obtained. In conclusion, this study not only provides novel gene source and germplasms for wheat resistance breeding, but also laid a solid foundation for cloning of PmW6RS.
新型小麦-黑麦 6RS 小片段易位系的抗白粉病特性被开发出来，并且将抗白粉病基因 PmW6RS 物理定位到威宁黑麦的 6RS-0.58-0.66-bin 对应于 18.38 Mb 的位置。黑麦（Secale cereale L.，RR）含有可用于改良小麦的有价值基因。然而，大多数黑麦抗性基因尚未成功应用于小麦品种。通过开发小片段易位系鉴定新的黑麦抗性基因并将这些基因转移到小麦中，将使这些基因更易于用于小麦育种。在这项研究中，使用一组新的小麦-黑麦二体和端体附加系，将广谱白粉病抗性基因 PmW6RS 定位到黑麦染色体臂 6RS 上。为了进一步研究和利用 PmW6RS，通过 Coγ射线辐射开发了 164 个小麦-黑麦 6RS 易位系。幼苗期和成株期白粉病抗性分析表明，其中 106 个易位系具有抗性。使用 6RS 易位和缺失系构建了 6RS 的物理图谱，并将 PmW6RS 定位在 6RS-0.58-0.66-bin 中，该区间由标记 X6RS-3 和 X6RS-10 侧翼，对应于威宁黑麦基因组中 50.23-68.61 Mb 的物理间隔。共注释了 23 个与抗性相关的基因。9 个标记与 6RS-0.58-0.66-bin 共分离，可用于快速追踪携带 PmW6RS 的 6RS 片段。带有白粉病抗性的小片段易位系与小麦品种回交，获得了 39 个农艺上可接受的 6RS 小片段纯合易位系。总之，本研究不仅为小麦抗性育种提供了新的基因源和种质资源，而且为 PmW6RS 的克隆奠定了坚实的基础。
