Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.
Nanoscale. 2017 Jun 14;9(23):7822-7829. doi: 10.1039/c7nr01511a.
Surface-Enhanced Raman Scattering (SERS) is emerging as a promising strategy for the quantification of immunoglobulin G (IgG) due to its inherent high sensitivity and specificity; however, it remains challenging to integrate SERS detection with a microfluidic system in a simple, efficient and low-cost manner. Here, we report on a novel bifunctional plasmonic-magnetic particle-based immunoassay, in which plasmonic nanoparticles act as soluble SERS immunosubstrates, whereas magnetic particles are for promoting micromixing in a microfluidic chip. With this novel SERS immunosubstrate in conjunction with the unique microfluidic system, we could substantially reduce the assay time from 4 hours to 80 minutes as well as enhance the detection specificity by about 70% in comparison to a non-microfluidic immunoassay. Compared to previous microfluidic SERS systems, our strategy offers a simple microfluidic chip design with only one well for mixing, washing and detection.
表面增强拉曼散射（SERS）由于其固有的高灵敏度和特异性，正在成为一种很有前途的免疫球蛋白 G（IgG）定量策略；然而，以简单、高效和低成本的方式将 SERS 检测与微流控系统集成仍然具有挑战性。在这里，我们报告了一种新型的基于等离子体-磁性颗粒的双功能免疫分析，其中等离子体纳米粒子作为可溶性 SERS 免疫底物，而磁性颗粒用于促进微流控芯片中的微混合。通过这种新型 SERS 免疫底物和独特的微流控系统，与非微流控免疫分析相比，我们可以将检测时间从 4 小时缩短到 80 分钟，并且可以将检测特异性提高约 70%。与以前的微流控 SERS 系统相比，我们的策略提供了一种简单的微流控芯片设计，只有一个用于混合、洗涤和检测的孔。
