抑制细胞对单纯疱疹病毒特异性CTL诱导的调节作用。

The regulation of herpes simplex virus-specific CTL induction by suppressor cells.

作者信息

Horohov D W, Moore R N, Rouse B T

机构信息

Center for Drugs and Biologics, Division of Virology, Food and Drug Administration, Bethesda, MD 20892.

出版信息

Viral Immunol. 1987 Spring;1(1):55-68. doi: 10.1089/vim.1987.1.55.

DOI:10.1089/vim.1987.1.55

PMID:2855699

Abstract

The incubation of herpes simplex virus (HSV) immune murine splenocytes with HSV antigens induced suppressor cells which inhibited HSV-specific cytotoxic T lymphocyte (CTL) induction. The cell mediating the suppression was identified as a Thy 1+ Lyt 2+ I-J+ cell. The induction of this suppressor cell required the participation of at least three leukocyte populations. That is, depleting the cultures of either Lyt 1+ or Lyt 2+ splenocytes resulted in a failure to induce suppressor cell activity. Likewise the removal of macrophage-like antigen-presenting cells (APC), in particular I-A- I-J+ APC, abolished suppressor-cell induction. Though the Lyt 2+ I-J+ cells had to be provided by HSV-immune mice, both the APC and the Lyt 1+ cells could be provided by HSV-naive mice. Though the induction of the suppressor cell was virus specific, its action was nonspecific as evidenced by the suppression of influenza-specific CTL induction. The implication of our results for the understanding and manipulation of herpesvirus disease is briefly discussed.

摘要

将单纯疱疹病毒（HSV）免疫的小鼠脾细胞与HSV抗原一起培养可诱导出抑制细胞，这些抑制细胞会抑制HSV特异性细胞毒性T淋巴细胞（CTL）的诱导。介导这种抑制作用的细胞被鉴定为Thy 1+ Lyt 2+ I-J+细胞。这种抑制细胞的诱导至少需要三个白细胞群体的参与。也就是说，去除Lyt 1+或Lyt 2+脾细胞的培养物会导致无法诱导抑制细胞活性。同样，去除巨噬细胞样抗原呈递细胞（APC），特别是I-A- I-J+ APC，会消除抑制细胞的诱导。虽然Lyt 2+ I-J+细胞必须由HSV免疫的小鼠提供，但APC和Lyt 1+细胞都可以由未接触过HSV的小鼠提供。虽然抑制细胞的诱导具有病毒特异性，但其作用是非特异性的，这在对流感特异性CTL诱导的抑制中得到了证明。我们简要讨论了这些结果对理解和控制疱疹病毒疾病的意义。