Tang Wen-Zhao, Wang Ying-Ai, Gao Tian-Yang, Wang Xiao-Jing, Zhao Yun-Xue
Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Jinan 250062, China; State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Jinan 250062, China.
Chin J Nat Med. 2017 May;15(5):384-391. doi: 10.1016/S1875-5364(17)30059-6.
The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C (1) and five known C-geranylated flavanones (2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CHCl extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by HO. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone (3) and paucatalinone A (5) were the potent anti-aging agents in the premature senescent 2BS cells induced by HO and the C-geranyl substituent may be an important factor because of its lipophilic character.
兰考泡桐的果实作为一种中国民间草药，用于治疗肠炎、扁桃体炎、支气管炎和痢疾等。我们之前的研究已鉴定出在肺癌A549细胞中具有明显抗增殖作用的新的C-香叶基化黄烷酮。在本研究中，分离得到了一种新的C-香叶基化黄酮，泡桐素C（1）和五种已知的C-香叶基化黄烷酮（2-6）。此外，通过HPLC-DAD-ESI-MS/MS联用技术从兰考泡桐的CHCl提取物中总共检测到34种C-香叶基化黄酮类化合物。此外，用H₂O₂诱导的早衰2BS细胞在体外评估了分离化合物的抗衰老作用。植物化学结果表明，兰考泡桐是丰富的C-香叶基化黄酮类化合物的天然来源。双黄酮（3）和泡桐素A（5）是H₂O₂诱导的早衰2BS细胞中的有效抗衰老剂，并且C-香叶基取代基因其亲脂性可能是一个重要因素。
