Grarup J, Hasselager E, Hjelm M
Scand J Clin Lab Invest. 1977 Feb;37(1):85-8.
The heat production (HP) of glucose deprived human red blood cells was measured, using glucose, adenine and inosine as substrates. Inosine induced a significantly higher HP than glucose and adenine induced no significant HP. At low pH the HP of glucose decreased more than that of inosine, corresponding to an equally lowered lactate production. The results indicate that it should be possible to use the system developed to study the functional state both of the complete glycolytic system and the lower part of it in intact red blood cells during various clinical conditions.
以葡萄糖、腺嘌呤和肌苷作为底物,测量了葡萄糖缺乏的人体红细胞的产热(HP)。肌苷诱导的产热显著高于葡萄糖,而腺嘌呤未诱导出显著的产热。在低pH值下,葡萄糖的产热比肌苷下降得更多,这与乳酸生成同样减少相对应。结果表明,利用所开发的系统来研究完整红细胞在各种临床状况下整个糖酵解系统及其较低部分的功能状态应该是可行的。