Law Jodi Woan-Fei, Ser Hooi-Leng, Duangjai Acharaporn, Saokaew Surasak, Bukhari Sarah I, Khan Tahir M, Ab Mutalib Nurul-Syakima, Chan Kok-Gan, Goh Bey-Hing, Lee Learn-Han
Novel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, Malaysia.
Division of Physiology, School of Medical Sciences, University of PhayaoPhayao, Thailand.
Front Microbiol. 2017 May 16;8:877. doi: 10.3389/fmicb.2017.00877. eCollection 2017.
MUSC 93J, a novel strain isolated from mangrove forest soil located at Sarawak, Malaysia. The bacterium was noted to be Gram-positive and to form light yellow aerial and vivid yellow substrate mycelium on ISP 2 agar. The polyphasic approach was used to determine the taxonomy of strain MUSC 93J and the strain showed a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus . Phylogenetic and 16S rRNA gene sequence analysis indicated that closely related strains include NBRC 13059 (99.2% sequence similarity), NBRC 13063 (99.1%), and NRRL 5799 (99.1%). The DNA-DNA relatedness values between MUSC 93J and closely related type strains ranged from 14.4 ± 0.1 to 46.2 ± 0.4%. The comparison of BOX-PCR fingerprints indicated MUSC 93J exhibits a unique DNA profile. The genome of MUSC 93J consists of 7,015,076 bp. The DNA G + C content was determined to be 69.90 mol%. The extract of strain MUSC 93J was demonstrated to exhibit potent antioxidant activity via ABTS, metal chelating, and SOD assays. This extract also exhibited anticancer activity against human colon cancer cell lines without significant cytotoxic effect against human normal colon cells. Furthermore, the chemical analysis of the extract further emphasizes the strain is producing chemo-preventive related metabolites. Based on this polyphasic study of MUSC 93J, it is concluded that this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is MUSC 93J (= DSM 102042 = MCCC 1K02298).
MUSC 93J是从马来西亚砂拉越的红树林土壤中分离出的一种新型菌株。该细菌为革兰氏阳性菌，在ISP 2琼脂上形成浅黄色气生菌丝体和鲜黄色基内菌丝体。采用多相分类法确定菌株MUSC 93J的分类地位，该菌株表现出一系列与该属成员一致的系统发育和化学分类学特征。系统发育和16S rRNA基因序列分析表明，与之密切相关的菌株包括NBRC 13059（序列相似性99.2%）、NBRC 13063（99.1%）和NRRL 5799（99.1%）。MUSC 93J与密切相关的模式菌株之间的DNA-DNA相关性值在14.4±0.1%至46.2±0.4%之间。BOX-PCR指纹图谱比较表明，MUSC 93J呈现出独特的DNA图谱。MUSC 93J的基因组由7,015,076 bp组成。DNA G + C含量测定为69.90 mol%。通过ABTS、金属螯合和超氧化物歧化酶（SOD）测定，证明菌株MUSC 93J的提取物具有强大的抗氧化活性。该提取物还对人结肠癌细胞系表现出抗癌活性，而对人正常结肠细胞无明显细胞毒性作用。此外，提取物的化学分析进一步表明该菌株产生与化学预防相关的代谢产物。基于对MUSC 93J的多相研究，得出该菌株代表一个新物种的结论，并提议将其命名为sp. nov.。模式菌株为MUSC 93J（= DSM 102042 = MCCC 1K02298）。
