Ser Hooi-Leng, Ab Mutalib Nurul-Syakima, Yin Wai-Fong, Chan Kok-Gan, Goh Bey-Hing, Lee Learn-Han
Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia Bandar Sunway, Malaysia.
UKM Medical Molecular Biology Institute-UKM Medical Centre, Universiti Kebangsaan Malaysia Kuala Lumpur, Malaysia.
Front Microbiol. 2015 Dec 16;6:1398. doi: 10.3389/fmicb.2015.01398. eCollection 2015.
Streptomyces pluripotens MUSC 137 was isolated from mangrove soil obtained from Tanjung Lumpur, Pahang, Malaysia. We investigated the phylogenetic, genomic, biochemical, and phenotypic characteristics of this strain. Uniquely adapted microorganisms from mangrove habitats have previously yielded compounds of biopharmaceutical interest. In order to examine the bioactivities possessed by the strain, fermentation extract was prepared through solvent extraction method prior to bioactivities screenings. Antioxidant activity was examined via DPPH assay while the cytotoxic effect was assessed by means of examining the activity of the extract against selected human cancer cell lines, namely colon cancer cells (HCT-116, Caco-2, SW480, and HT-29), breast cancer cell (MCF-7), lung cancer cell (A549), prostate cancer cell (DU145), and cervical cancer cell (Ca Ski). The results revealed MUSC 137 possesses significant antioxidant activity and demonstrates cytotoxic effect against several cancer cell lines tested. The results indicated MCF-7 cells were most susceptible to the extract with the lowest IC50 (61.33 ± 17.10 μg/mL), followed by HCT-116 and A549. Additionally, selective index (SI) showed that MUSC 137 extract was less toxic against normal cell lines when compared to MCF-7 and HCT-116 cells. The extract was further subjected to chemical analysis using GC-MS and revealed the presence of deferoxamine and pyrrolizidines related compounds which may account for the antioxidant and cytotoxic properties observed.
多能链霉菌MUSC 137是从马来西亚彭亨州丹戎隆坡的红树林土壤中分离出来的。我们研究了该菌株的系统发育、基因组、生化和表型特征。此前，来自红树林栖息地的独特适应性微生物已产生具有生物制药意义的化合物。为了检测该菌株所具有的生物活性，在进行生物活性筛选之前，通过溶剂萃取法制备了发酵提取物。通过DPPH法检测抗氧化活性，同时通过检测提取物对选定的人类癌细胞系的活性来评估细胞毒性作用，这些癌细胞系包括结肠癌细胞（HCT-116、Caco-2、SW480和HT-29）、乳腺癌细胞（MCF-7）、肺癌细胞（A549）、前列腺癌细胞（DU145）和宫颈癌细胞（Ca Ski）。结果显示，MUSC 137具有显著的抗氧化活性，并对几种测试的癌细胞系表现出细胞毒性作用。结果表明，MCF-7细胞对提取物最敏感，IC50最低（61.33±17.10μg/mL），其次是HCT-116和A549。此外，选择性指数（SI）表明，与MCF-7和HCT-116细胞相比，MUSC 137提取物对正常细胞系的毒性较小。该提取物进一步通过气相色谱-质谱联用（GC-MS）进行化学分析，结果显示存在去铁胺和与吡咯里西啶相关的化合物，这可能解释了所观察到的抗氧化和细胞毒性特性。
