Lobysheva Irina I, Eeckhoudt Sandrine van, Zotti Flavia Dei, Rifahi Ahmad, Pothen Lucie, Beauloye Christophe, Balligand Jean-Luc
Institut de Recherche Experimentale et Clinique (IREC), Pole of Pharmacology and Therapeutics (FATH), Université Catholique de Louvain, Brussels, Belgium.
Pole of Cardiovascular Research (CARD), and Departments of Internal Medicine and Cardiovascular Diseases, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium.
Data Brief. 2017 May 12;13:46-52. doi: 10.1016/j.dib.2017.05.019. eCollection 2017 Aug.
The data presented in this article are associated with the research article entitled "Heme-Nitrosylated Hemoglobin and Oxidative Stress in Women Consuming Combined Contraceptives. Clinical Application of the EPR Spectroscopy" (Lobysheva et al., 2017 [1]), and describe the characteristics of redox status in blood, as well as biochemical and clinical parameters of young female subjects consuming (or not) contraceptive pills (CP). Erythrocyte concentration of reduced thiols reflecting erythrocyte redox capacity was measured before and after sample deproteinization by electron paramagnetic resonance spectroscopy (EPR) using a nitroxide biradical spin probe specifically interacting with reduced thiols; additional data were obtained by a colorimetric method using Ellman׳s reagents in the same samples. The products of nitric oxide oxidation, nitrite and total NO (in presence of nitrate reductase) were measured in the plasma of study subjects by a colorimetric assay based on the detection of red-violet colored azo dye after reaction of nitrite with the Griess reagent. Biochemical and clinical parameters reflective of cardiovascular risk factors (diastolic blood pressure, C-reactive protein, triglycerides and homocysteine concentrations in venous blood) were compared in subgroups of consumers of CP containing ethinyl estradiol and different types of synthetic progestogens. Parameters reflective of the integrity of the vasculature, - erythrocyte concentration of heme-nitrosylated hemoglobin (5-coordinate α-heme-Fe-NO, HbNO) measured directly by the EPR subtraction method; index of reactive hyperemia response (FRHI) measured by digital pulse tonometry using EndoPAT; oxidative vascular stress measured as total plasma peroxide concentration were compared in subgroups of young women taking CP containing ethinyl estradiol at different concentrations and for various durations.
本文所呈现的数据与题为《服用复方避孕药女性中的血红素亚硝基化血红蛋白与氧化应激。电子顺磁共振波谱的临床应用》（洛比舍娃等人，2017 [1]）的研究文章相关，描述了血液中氧化还原状态的特征，以及服用（或未服用）避孕药（CP）的年轻女性受试者的生化和临床参数。通过电子顺磁共振波谱（EPR），使用与还原型硫醇特异性相互作用的氮氧双自由基自旋探针，在样品脱蛋白前后测量反映红细胞氧化还原能力的还原型硫醇的红细胞浓度；通过使用埃尔曼试剂的比色法在相同样品中获得了额外数据。采用基于亚硝酸盐与格里斯试剂反应后检测红紫色偶氮染料的比色测定法，测定研究对象血浆中一氧化氮氧化产物、亚硝酸盐和总NO（在存在硝酸还原酶的情况下）。在含有乙炔雌二醇和不同类型合成孕激素的CP使用者亚组中，比较了反映心血管危险因素的生化和临床参数（舒张压、C反应蛋白、静脉血中甘油三酯和同型半胱氨酸浓度）。在服用不同浓度和不同持续时间含乙炔雌二醇CP的年轻女性亚组中，比较了反映血管系统完整性的参数——通过EPR减法直接测量的血红素亚硝基化血红蛋白（5配位α-血红素-Fe-NO，HbNO）的红细胞浓度；使用EndoPAT通过数字脉搏张力测量法测量的反应性充血反应指数（FRHI）；以总血浆过氧化物浓度衡量的氧化血管应激。
