Paul Sujay
Division of Plant Biology, Bose Institute, P 1/12 CIT Scheme VII M, Kolkata, 700054, India.
Laboratorio de Micología y Biotecnología, Universidad Nacional Agraria La Molina, Av. La Molina s/n, 12, Lima, Peru.
3 Biotech. 2017 Jun;7(2):103. doi: 10.1007/s13205-017-0734-5. Epub 2017 May 30.
MicroRNAs (miRNAs) are endogenous, short (~21-nucleotide), non-coding RNA molecules that play pivotal roles in plant growth, development, and stress response signaling. In this study using recently published draft genome sequence of a high-altitude plant maca (Lepidium meyenii Walp) and applying genome-wide computational-based approaches, a total of 62 potentially conserved miRNAs belonging to 28 families were identified and four (lme-miR160a, lme-miR164c, lme-miR 166a, and lme-miR 319a) of them further validated by RT-PCR. Deploying psRNATarget tool a total of 99 potential miRNA target transcripts were also identified in maca. Targets include a number of transcription factors like Squamosa promoter-binding, NAC, MYB, auxin response factor, APETALA, WRKY, and F-box protein. To the best of my knowledge, this is the first genome-based miRNA profiling of a high-altitude plant.
微小RNA（miRNA）是内源性的短链（约21个核苷酸）非编码RNA分子，在植物生长、发育及应激反应信号传导中发挥关键作用。在本研究中，利用最近公布的一种高海拔植物玛咖（Lepidium meyenii Walp）的基因组序列草图，并应用全基因组计算方法，共鉴定出属于28个家族的62个潜在保守miRNA，其中4个（lme-miR160a、lme-miR164c、lme-miR 166a和lme-miR 319a）通过逆转录聚合酶链反应（RT-PCR）进一步验证。利用psRNATarget工具，在玛咖中还共鉴定出99个潜在的miRNA靶转录本。靶标包括一些转录因子，如鳞状启动子结合蛋白、NAC、MYB、生长素反应因子、APETALA、WRKY和F-box蛋白。据我所知，这是首次对高海拔植物进行基于基因组的miRNA分析。
