Department of Pharmacy, Pharmacology and Postgraduate Medicine, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, UK.
J Pharm Pharmacol. 2019 Jan;71(1):38-45. doi: 10.1111/jphp.12742. Epub 2017 May 31.
A method was developed to analyze St John's wort (Hypericum perforatum L.) herb and preparations using inductively coupled plasma-optical emission spectroscopy (ICP-OES) to determine the quantity of 11 elements (Al, B, Ba, Ca, Cu, Fe, Mg, Mn, Ni, Sr and Zn).
This study includes the evaluation of digestion acids and calibration methods, as well as instrumental parameters such as choice of nebulizer and emission wavelength.
Two nebulizers (Conikal and SeaSpray) performed similarly for most elements, and two optimum wavelengths were determined for each element. Five acids were evaluated for the digestion of the Polish Certified Reference Material Tea Leaves (INCT-TL-1), while three were taken forward to use for the different St John's wort formulations (i.e. herb, capsule and tablet). A simple protocol using 5 ml HNO was sufficient in most cases; however, variability was observed for elements often bound in silicates (e.g. Al, Fe and Zn). An external weighted calibration was also found to be preferential over unweighted, and the use of standard addition affected some concentration values up to 20%.
Therefore, this paper presents the development and optimized method parameters to be used with ICP-OES that will allow the analysis of 11 key elements present in St John's wort herb and preparations.
开发了一种使用电感耦合等离子体-光学发射光谱法（ICP-OES）分析贯叶连翘（贯叶连翘 L.）草药及其制剂的方法，以测定 11 种元素（Al、B、Ba、Ca、Cu、Fe、Mg、Mn、Ni、Sr 和 Zn）的含量。
本研究包括评估消解酸和校准方法，以及仪器参数，如选择雾化器和发射波长。
对于大多数元素，两种雾化器（Conikal 和 SeaSpray）的性能相似，对于每个元素确定了两个最佳波长。评估了 5 种酸用于消解波兰认证参考材料茶叶（INCT-TL-1），其中 3 种酸用于不同的贯叶连翘制剂（即草药、胶囊和片剂）。在大多数情况下，使用 5ml HNO 进行简单的方案就足够了；然而，对于经常与硅酸盐结合的元素（如 Al、Fe 和 Zn），观察到了可变性。还发现外部加权校准优于非加权校准，标准添加的使用会影响高达 20%的某些浓度值。
因此，本文提出了开发和优化 ICP-OES 使用的方法参数，该方法可用于分析贯叶连翘草药及其制剂中存在的 11 种关键元素。
