[巴西帕拉州贝伦市儿童粪便中轮状病毒核酸电泳图谱的时间分布]

[Temporal distribution of electrophoretic profiles of nucleic acid from rotavirus in the feces of children in Belém, Pará, Brazil].

作者信息

Mascarenhas J D, Gabbay Y B, Freitas R B, Linhares A C

机构信息

Instituto Evandro Chagas, Fundação Serviços de Saúde Pública, Belém, PA, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 1988 Oct-Dec;83(4):415-9. doi: 10.1590/s0074-02761988000400004.

DOI:10.1590/s0074-02761988000400004

PMID:2856078

Abstract

Electrophoresis of rotavirus RNA in polyacrylamide gel was carried out by using faecal samples collected from children living in the peripheral area of Belém, Brazil. Typical rotavirus profiles (eleven bands) were noted in 46(76.7%) out of 60 ELISA rotavirus positive specimens. According to the electrophoretic patterns, 5(10.9%) were classified as subgroup I, while 41(89.1%) of them as subgroup II. Lacking of bands 10 and 11 in 14(23.3%) samples did not allow us to classify their electropherotypes. The profile coded as "1N2L" was the predominant one, accounting for 65.2% of the classified strains.

摘要

采用从巴西贝伦周边地区儿童采集的粪便样本，在聚丙烯酰胺凝胶中对轮状病毒RNA进行电泳分析。在60份ELISA轮状病毒阳性标本中，46份（76.7%）呈现典型的轮状病毒图谱（11条带）。根据电泳图谱，5份（10.9%）被归类为I亚组，而其中41份（89.1%）为II亚组。14份（23.3%）样本中缺少第10和11条带，无法对其电泳型进行分类。编码为“1N2L”的图谱是主要类型，占已分类菌株的65.2%。