Mezi Silvia, Chiappetta Caterina, Carletti Raffaella, Nardini Andrea, Cortesi Enrico, Orsi Errico, Piesco Gabriele, Di Gioia Cira
Department of Radiological Oncological and Pathological Sciences, "Sapienza" University of Rome, Rome, Italy.
Department of Surgical Science, "Sapienza" University of Rome, Rome, Italy.
Head Neck. 2017 Sep;39(9):1806-1818. doi: 10.1002/hed.24838. Epub 2017 May 31.
During epithelial-to-mesenchymal transition, cancer cells lose adhesion capacity gaining migratory properties. The role of the process on prognosis has been evaluated in 50 cases of laryngeal carcinoma.
E-cadherin, N-cadherin, β-catenin, α-catenin, γ-catenin, caveolin-1, and vimentin immunohistochemical expression were evaluated using a double score based on staining intensity and cellular localization.
Cytoplasmic E-cadherin and α/γ catenin staining were associated with a decrease in survival, cytoplasmic β-catenin was associated with advanced stage, and N-cadherin and vimentin expression were associated with poor differentiation and tumor relapse. On the basis of cancer cells, epithelial or mesenchymal morphological and immunophenotypic similarity we identified 4 main subgroups correlated with a transition to a more undifferentiated phenotype, which have a different pattern of relapse and survival.
The negative prognostic role of epithelial-to-mesenchymal transition has been confirmed and a predictive role in glottic tumors has been suggested, leading us to propose epithelial-to-mesenchymal transition as an additional adverse feature in laryngeal carcinoma.
在上皮-间质转化过程中，癌细胞失去黏附能力并获得迁移特性。已在50例喉癌病例中评估了该过程对预后的作用。
基于染色强度和细胞定位，使用双评分法评估E-钙黏蛋白、N-钙黏蛋白、β-连环蛋白、α-连环蛋白、γ-连环蛋白、小窝蛋白-1和波形蛋白的免疫组化表达。
细胞质E-钙黏蛋白和α/γ连环蛋白染色与生存率降低相关，细胞质β-连环蛋白与晚期相关，N-钙黏蛋白和波形蛋白表达与低分化和肿瘤复发相关。基于癌细胞的上皮或间质形态及免疫表型相似性，我们确定了4个主要亚组，它们与向更未分化表型的转变相关，且具有不同的复发和生存模式。
上皮-间质转化的负面预后作用已得到证实，并提示其在声门肿瘤中具有预测作用，这使我们提出上皮-间质转化是喉癌的另一个不良特征。
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