Sorgini Alana, Kim Hugh Andrew Jinwook, Zeng Peter Y F, Shaikh Mushfiq Hassan, Mundi Neil, Ghasemi Farhad, Di Gravio Eric, Khan Halema, MacNeil Danielle, Khan Mohammed Imran, Mendez Adrian, Yoo John, Fung Kevin, Lang Pencilla, Palma David A, Mymryk Joe S, Barrett John W, Patel Krupal B, Boutros Paul C, Nichols Anthony C
Department of Otolaryngology, Head and Neck Surgery, University of Western Ontario, London, ON N6A 5W9, Canada.
Department of General Surgery, University of Western Ontario, London, ON N6A 5C5, Canada.
Cancers (Basel). 2020 Dec 31;13(1):105. doi: 10.3390/cancers13010105.
Laryngeal squamous cell carcinoma (LSCC) from different subsites have distinct presentations and prognosis. In this study, we carried out a multiomic comparison of LSCC subsites. The Cancer Genome Atlas (TCGA) LSCC cohort was analyzed in the R statistical environment for differences between supraglottic and glottic cancers in single nucleotide variations (SNVs), copy number alterations (CNAs), mRNA abundance, protein abundance, pathway overrepresentation, tumor microenvironment (TME), hypoxia status, and patient outcome. Supraglottic cancers had significantly higher overall and smoking-associated SNV mutational load. Pathway analysis revealed upregulation of muscle related pathways in glottic cancer and neural pathways in supraglottic cancer. Proteins involved in cancer relevant signaling pathways including PI3K/Akt/mTOR, the cell cycle, and PDL1 were differentially abundant between subsites. Glottic and supraglottic tumors have different molecular profiles, which may partially account for differences in presentation and response to therapy.
来自不同亚部位的喉鳞状细胞癌（LSCC）具有不同的表现和预后。在本研究中，我们对LSCC亚部位进行了多组学比较。在R统计环境中分析了癌症基因组图谱（TCGA）LSCC队列，以比较声门上癌和声门癌在单核苷酸变异（SNV）、拷贝数改变（CNA）、mRNA丰度、蛋白质丰度、通路富集、肿瘤微环境（TME）、缺氧状态和患者预后方面的差异。声门上癌的总体和与吸烟相关的SNV突变负荷显著更高。通路分析显示，声门癌中肌肉相关通路上调，声门上癌中神经通路上调。参与癌症相关信号通路（包括PI3K/Akt/mTOR、细胞周期和PDL1）的蛋白质在亚部位之间的丰度存在差异。声门癌和声门上癌具有不同的分子特征，这可能部分解释了临床表现和对治疗反应的差异。
