根际感染真菌蜜环菌属生物种间核糖体DNA的变异

VARIATION IN RIBOSOMAL DNA AMONG BIOLOGICAL SPECIES OF ARMILLARIA, A GENUS OF ROOT-INFECTING FUNGI.

作者信息

Anderson James B, Bailey Steven S, Pukkila Patricia J

机构信息

Department of Botany, Erindale College, University of Toronto, Mississauga, Ontario, L5L 1C6, CANADA.

Department of Biology, Curriculum in Genetics, University of North Carolina, Chapel Hill, NC, USA, 27599-3280.

出版信息

Evolution. 1989 Dec;43(8):1652-1662. doi: 10.1111/j.1558-5646.1989.tb02615.x.

DOI:10.1111/j.1558-5646.1989.tb02615.x

PMID:28564337

Abstract

Armillaria is a genus of root-infecting fungi composed of several biological species in North America and Europe. To examine relatedness among biological species, ribosomal DNA (rDNA) from one isolate was cloned and rDNAs from 30 isolates were mapped for eight restriction enzymes. The positions of the large (26S) and small (18S) rRNA cistrons were found by Northern hybridizations of total cellular RNA with rDNA subclones and by alignment of maps with conserved restriction sites present in rRNA genes of other fungi. Nine restriction-site and two length polymorphisms were observed. Eight North American (Roman numerals) and five European (species epithets) biological species could be placed in six classes with respect to rDNA maps (rDNA class 1: I and A. ostoyae; class 2: II; class 3: A. borealis; class 4: V, IX, and X; class 5: III, VII, A. lutea, and A. cepistipes; and class 6: VI and A. mellea). Most, but not all, polymorphisms were in intergenic regions.

摘要

蜜环菌属是一类能感染根部的真菌，在北美和欧洲由几个生物种组成。为了研究生物种之间的亲缘关系，对一个分离株的核糖体DNA（rDNA）进行了克隆，并对30个分离株的rDNA用8种限制性内切酶进行了图谱绘制。通过用rDNA亚克隆对总细胞RNA进行Northern杂交，以及将图谱与其他真菌rRNA基因中存在的保守限制性位点进行比对，确定了大（26S）和小（18S）rRNA顺反子的位置。观察到9个限制性位点多态性和2个长度多态性。就rDNA图谱而言，8个北美（罗马数字）和5个欧洲（种加词）生物种可分为6类（rDNA第1类：I和奥氏蜜环菌；第2类：II；第3类：北方蜜环菌；第4类：V、IX和X；第5类：III、VII、黄蜜环菌和鳞柄蜜环菌；第6类：VI和蜜环菌）。大多数（但不是全部）多态性存在于基因间区域。