Yasuno H, Yamanishi K
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
Hum Cell. 1988 Jun;1(2):162-70.
The usual methods of skin culture composed of organ culture, explant culture and cell culture were described. In organ culture of normal human skin, some blister diseases models have been used for the study of the mechanisms for producing the skin damage in pemphigus, bullous pemphigoid and epidermolysis bullosa hereditaria. Recent advances in epidermal cell culture system have furnished a potent tool for the study of keratinization at the molecular level. In the present status on tissue culture of human skin, these applications to clinical and laboratory investigations were discussed.
描述了皮肤培养的常用方法，包括器官培养、外植体培养和细胞培养。在正常人皮肤的器官培养中，一些水疱病模型已被用于研究天疱疮、大疱性类天疱疮和遗传性大疱性表皮松解症中皮肤损伤产生的机制。表皮细胞培养系统的最新进展为在分子水平上研究角质化提供了有力工具。在人类皮肤组织培养的现状下，讨论了这些方法在临床和实验室研究中的应用。
