Timár J, Nagy K, Lapis K
1st Inst. of Pathol. and Exptl. Canc. Res., Semmelweis Med. Univ., Budapest, Hungary.
Histol Histopathol. 1986 Jan;1(1):43-7.
The AIDS associated HTLV-III virus infected H9 cells were extensively studied using light, scanning and transmission electronmicroscopy. It was demonstrated that the morphological features of HTLV-III are different from the C-type particles and are similar to those of lentiviridae. For immunological identification high titer pre-AIDS patient sera served as the anti-HTLV-III envelope antibody source. The immunoelectronmicroscopic method was able to identify the viral envelope antigen on the surface of infected cells and in certain areas of the viral envelope. This is the first application of immunoelectronmicroscopy for the identification of the HTLV-III virus.
利用光学显微镜、扫描电子显微镜和透射电子显微镜对与艾滋病相关的人嗜T淋巴细胞病毒III型（HTLV-III）感染的H9细胞进行了广泛研究。结果表明，HTLV-III的形态特征不同于C型颗粒，与慢病毒科的形态特征相似。为进行免疫鉴定，以艾滋病前期患者的高滴度血清作为抗HTLV-III包膜抗体来源。免疫电子显微镜方法能够鉴定感染细胞表面和病毒包膜某些区域的病毒包膜抗原。这是免疫电子显微镜首次应用于HTLV-III病毒的鉴定。
