Chen Zhe, Tao Ze-Zhang, Zhou Xu-Hong, Wu Ting-Ting, Ye Lin-Feng
Department of Otolaryngology Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430072, P.R. China.
Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Exp Ther Med. 2017 May;13(5):2405-2410. doi: 10.3892/etm.2017.4237. Epub 2017 Mar 20.
Allergic rhinitis (AR) is a chronic allergic airway disease that has become a significant global public health issue. Sinomenine (SN), a natural phytochemical found in , showed anti-inflammatory and immunosuppressive effect in previous studies. In order to explore the role of SN in the treatment of AR, mice were sensitized and challenged by ovalbumin (OVA) to establish an AR mouse model. SN was administered to AR mice orally, and compared with dexamethasone treatment as a positive control. Nasal symptoms and histopathological changes were used to evaluate the effect of SN treatment in the AR mice model. In addition, the levels of anti-OVA specific IgE and various cytokines in the serum were measured by enzyme-linked immunosorbent assay, while the levels of transforming growth factor-β (TGF-β) in the mucosa were also detected by western blot analysis and reverse transcription-quantitative polymerase chain reaction. AR mice that received SN treatment had reduced symptom scores and milder eosinophil infiltration. The serum levels of anti-OVA specific IgE and interleukin-4 significantly decreased following SN treatment. Furthermore, TGF-β expression levels in the serum and nasal mucosa tissue in AR mice increased when compared with those in AR mice without treatment. In conclusion, SN treatment alleviated the symptoms of AR in mice and had an immunosuppressive effect on AR, which may result from the upregulation of TGF-β.
变应性鼻炎（AR）是一种慢性变应性气道疾病，已成为全球重大的公共卫生问题。青藤碱（SN）是一种存在于[植物名称未给出]中的天然植物化学物质，在先前的研究中显示出抗炎和免疫抑制作用。为了探究SN在AR治疗中的作用，通过卵清蛋白（OVA）对小鼠进行致敏和激发，以建立AR小鼠模型。将SN口服给予AR小鼠，并与作为阳性对照的地塞米松治疗进行比较。采用鼻症状和组织病理学变化来评估SN治疗对AR小鼠模型的效果。此外，通过酶联免疫吸附测定法检测血清中抗OVA特异性IgE和各种细胞因子的水平，同时通过蛋白质印迹分析和逆转录-定量聚合酶链反应检测黏膜中转化生长因子-β（TGF-β）的水平。接受SN治疗的AR小鼠症状评分降低，嗜酸性粒细胞浸润较轻。SN治疗后，血清中抗OVA特异性IgE和白细胞介素-4的水平显著降低。此外，与未治疗的AR小鼠相比，AR小鼠血清和鼻黏膜组织中的TGF-β表达水平升高。总之，SN治疗减轻了小鼠AR的症状，并对AR具有免疫抑制作用，这可能是由于TGF-β上调所致。
