Hjeresen D L, Brief D J, Amend D L, Dorsa D M, Woods S C
Biophysics/Neurobiology Group, Los Alamos National Laboratory, NM 87545.
Peptides. 1988;9 Suppl 1:193-200. doi: 10.1016/0196-9781(88)90244-6.
Administration of AVP and related peptide fragments following ethanol (EtOH) administration has been shown to enhance retention of tolerance to ethanol. The present studies were designed specifically to: (1) examine the influence of AVP given concurrently with EtOH on the development of tolerance to the ataxic and hypothermic effects of EtOH in Long-Evans rats, and (2) to determine if tolerance to these effects develops in Brattleboro rats which are deficient in AVP. In Experiment 1, EtOH (2.5 g/kg, 15% v/v) was administered IP to 2 groups of rats in combination with a SC injection of either AVP (6 micrograms/kg) or an equal volume of saline. Two additional control groups received IP saline injections in combination with either saline or AVP. After 13 days, EtOH-treated rats were significantly more tolerant than saline-treated animals. AVP significantly increased the hypothermic and ataxic effects of EtOH and failed to enhance tolerance development. AVP delayed the extinction of tolerance to the hypothermic (but not the ataxic) effects of ethanol when administered during the extinction phase to rats previously treated with EtOH. In Experiment 2, Brattleboro rats were injected with EtOH or an equivalent volume of saline and tested for ataxia and hypothermia. Rats receiving EtOH failed to demonstrate significant tolerance to either effect of ethanol after 12 treatment days.
在给予乙醇（EtOH）后给予抗利尿激素（AVP）及相关肽片段已被证明可增强对乙醇耐受性的保持。本研究专门设计用于：（1）研究与EtOH同时给予AVP对Long-Evans大鼠对EtOH共济失调和体温过低效应耐受性发展的影响，以及（2）确定在缺乏AVP的Brattleboro大鼠中是否会产生对这些效应的耐受性。在实验1中，将EtOH（2.5 g/kg，15% v/v）腹腔注射给2组大鼠，并皮下注射AVP（6微克/千克）或等体积的生理盐水。另外两个对照组接受腹腔注射生理盐水并分别联合注射生理盐水或AVP。13天后，经EtOH处理的大鼠比经生理盐水处理的动物耐受性明显更强。AVP显著增加了EtOH的体温过低和共济失调效应，并且未能增强耐受性的发展。当在消退期给先前用EtOH处理过的大鼠注射AVP时，它延迟了对乙醇体温过低（但不是共济失调）效应耐受性的消退。在实验2中，给Brattleboro大鼠注射EtOH或等体积的生理盐水，并测试其共济失调和体温过低情况。接受EtOH的大鼠在12个治疗日后对乙醇的任何一种效应均未表现出明显的耐受性。
