Yanthan J Sureni, Kehie Mechuselie, Kumaria Suman, Tandon Pramod
Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, Shillong, 793022, India.
Post Graduate Department of Environmental Science, Patkai Christian College (Autonomous), Chumukedima, Seithekema, Dimapur, 797103, India.
3 Biotech. 2017 Jun;7(2):124. doi: 10.1007/s13205-017-0777-7. Epub 2017 May 31.
An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmannii Vahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard medium (MS with 50 mg/l citric acid and 10 mg/l ascorbic acid) supplemented with 4 mg/l 6-benzylaminopurine (BAP) and 4 mg/l α-naphthalene acetic acid (NAA) followed by 26.8 ± 1.4 shoots in one-fourth strength SM fortified with 4 mg/l kinetin (KN) and 4 mg/l NAA. The well-developed plantlets with shoots and roots were potted in small plastic glasses filled with a mixture of sand and farmyard manure (3:1); these plantlets when transferred to a glasshouse for hardening and acclimatization showed 90% survival.
已从印度东北部的食虫植物布尔曼茅膏菜的茎尖开发出一种高效的体外再生方案。使用了各种植物生长调节剂来研究它们在诱导多芽和根方面的功效。在各种处理中，在添加了4 mg/l 6-苄基腺嘌呤（BAP）和4 mg/l α-萘乙酸（NAA）的四分之一强度标准培养基（含有50 mg/l柠檬酸和10 mg/l抗坏血酸的MS培养基）中观察到最多的芽（28.8 ± 1.5）和根（9.7 ± 0.6），其次是在添加了4 mg/l激动素（KN）和4 mg/l NAA的四分之一强度标准培养基中观察到26.8 ± 1.4个芽。将发育良好的带芽和根的小植株种植在装有沙子和农家肥混合物（3:1）的小塑料杯中；这些小植株转移到温室中进行硬化和驯化时，存活率为90%。
