Parzymies Marzena, Głębocka Katarzyna, Pogorzelec Magdalena, Banach-Albińska Barbara, Świstowska Alicja, Arciszewski Michał
Institute of Horticultural Production, University of Life Sciences in Lublin, Głęboka 28, 20-612 Lublin, Poland.
Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
Genes (Basel). 2025 Aug 25;16(9):1003. doi: 10.3390/genes16091003.
Tissue culture might be a method supplementing traditional plant propagation in various fields, like agriculture, medicine, industry, and the active conservation of plant species. For the purpose of plant restoration, it is important that the obtained progenies are identical with the mother plants to ensure the true-to-typeness of the future population.
In the present study, the stability of regenerants obtained in vitro through phenotypic and genetic analysis was estimated. Clones of aldrovanda plants were cultivated in tissue culture in the 1/10 MS liquid medium under the same conditions for over a year, with five weeks of subculturing.
It was observed that two clones formed plants that displayed atypical growth structures, the shoots were shorter with many lateral shoots, and they had a lower fresh weight. They also formed fewer and smaller snap-traps, which, in the case of carnivorous plants, determines the capability of catching prey. The 35 in vitro regenerated plants and 5 specimens obtained from the natural habitat were subjected to genetic analyses with two molecular markers: start codon targeted (SCoT) polymorphism and sequence-related amplified polymorphism (SRAP). Despite the visible morphological variants, the genetic stability of all the regenerants with the individuals from natural stands was confirmed. All of them were monomorphic except three bands that were obtained for reference, where individuals were amplified with SCoT28 and me12-em13 SRAP primers.
As shown in the presented research, it might be recommended to use different methods to evaluate the stability of in vitro cultivated plants.
组织培养可能是一种补充传统植物繁殖方法的手段，可应用于农业、医学、工业等各个领域以及植物物种的积极保护。为了植物恢复的目的，重要的是所获得的后代与母本植物相同，以确保未来种群的典型性。
在本研究中，通过表型和遗传分析评估了体外获得的再生植株的稳定性。轮叶狸藻植物的克隆在1/10 MS液体培养基中于相同条件下进行组织培养一年多，每五周继代培养一次。
观察到两个克隆形成的植株表现出非典型的生长结构，茎较短且有许多侧枝，鲜重较低。它们还形成了更少更小的捕虫夹，对于食虫植物来说，这决定了捕捉猎物的能力。对35株体外再生植株和5株从自然生境获得的标本，使用两种分子标记进行遗传分析：起始密码子靶向（SCoT）多态性和序列相关扩增多态性（SRAP）。尽管存在明显的形态变异，但所有再生植株与来自天然林分的个体的遗传稳定性得到了证实。除了使用SCoT28和me12-em13 SRAP引物对个体进行扩增得到的三条用于参考的条带外，所有植株均为单态。
如本研究所显示的，可能建议使用不同方法来评估体外培养植物的稳定性。
