Kim Hyeonsoo, Park Soohyun, Cho Sukhyeong, Yang Jeongmo, Jeong Kijun, Park Jinbyung, Lee Jinwon
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea.
Korea C1 Gas Refinery R&D Center, Sogang University, Seoul, 121-742, Republic of Korea.
J Ind Microbiol Biotechnol. 2017 Sep;44(9):1301-1311. doi: 10.1007/s10295-017-1945-9. Epub 2017 Jun 1.
Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 °C, but the reaction temperature was optimal at 30 °C. Enhanced conversion efficiency was obtained by supplying 0.05 g/L of Tween 80 at pH 7.5. Under these optimal conditions, recombinant C. glutamicum produced 0.28 mM of 9-(nonanoyloxy) nonanoic acid with a 75.6% (mol/mol) conversion yield in 2 h. This is the first report on the biotransformation of 10-ketostearic acid to 9-(nonanoyloxy) nonanoic acid with a recombinant whole-cell C. glutamicum-based biocatalyst and the results demonstrate the feasibility of using C. glutamicum as a whole-cell biocatalyst.
在此，设计了表达来自恶臭假单胞菌KT2440的拜耳-维利格单加氧酶的谷氨酸棒杆菌ATCC13032，用于从10-酮硬脂酸生产9-(壬酰氧基)壬酸。发现包括培养温度和反应温度、去污剂类型和pH在内的多种参数可提高生物转化效率。使用重组谷氨酸棒杆菌全细胞从10-酮硬脂酸生产9-(壬酰氧基)壬酸的最佳培养温度为15℃，但反应温度在30℃时最佳。在pH 7.5下供应0.05 g/L的吐温80可提高转化效率。在这些最佳条件下，重组谷氨酸棒杆菌在2小时内产生了0.28 mM的9-(壬酰氧基)壬酸，转化率为75.6%（摩尔/摩尔）。这是关于使用重组全细胞谷氨酸棒杆菌生物催化剂将10-酮硬脂酸生物转化为9-(壬酰氧基)壬酸的首次报道，结果证明了使用谷氨酸棒杆菌作为全细胞生物催化剂的可行性。
