Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO (Stress Environnementaux et BIOsurveillance des milieux aquatiques), UFR Sciences Exactes et Naturelles, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, Reims, France.
Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2 60550, Verneuil-en-Halatte, France.
Environ Sci Pollut Res Int. 2018 Apr;25(12):11252-11263. doi: 10.1007/s11356-017-9256-2. Epub 2017 May 31.
The global dynamic spread of chemical contamination through the aquatic environment calls for the development of biomarkers of interest. Reproduction is a key element to be considered because it is related to the sustainability of species. Spermatogenesis is a complex process that leads to the formation of mature germ cells, whose steps and impairments need to be finely described in ecotoxicological analyses. The physiological process has been commonly described by histological analyses of gonads in different taxa. In the present paper, we describe the development of a novel technique to characterize spermatogenesis based on the analysis of the DNA content of germ cells by flow cytometry, using a DNA-intercalating agent. This new biomarker, referred to as an index of sexual maturity, proved relevant to describe the seasonal reproductive cycle of the zebra mussel, Dreissena polymorpha (Pallas, 1771), used as a sentinel species in the biomonitoring of continental waters and sensitive to highlight the reprotoxicity of carbamazepine (an anti-epileptic pharmaceutical) tested under ecosystemic conditions (mesocosms).
全球范围内化学污染物通过水环境污染的动态扩散，需要开发有意义的生物标志物。因为这与物种的可持续性有关，所以繁殖是一个需要考虑的关键因素。精子发生是一个复杂的过程，导致成熟的生殖细胞的形成，其步骤和损伤需要在生态毒理学分析中进行精细描述。生理过程通常通过对不同分类群的性腺进行组织学分析来描述。在本文中，我们描述了一种新技术的发展，该技术基于使用 DNA 嵌入剂通过流式细胞术分析生殖细胞的 DNA 含量来表征精子发生。这个新的生物标志物，称为性成熟指数，被证明与斑马贻贝（Dreissena polymorpha）的季节性生殖周期有关，斑马贻贝被用作大陆水域生物监测的指示物种，对在生态系统条件下（中间容器）测试的卡马西平（一种抗癫痫药物）的生殖毒性敏感。
