Zunun-Perez A Y, Guevara-Figueroa T, Jimenez-Garcia S N, Feregrino-Perez A A, Gautier F, Guevara-Gonzalez R G
Laboratorio de Biosistemas, Facultad de Ingenieria, Campus Amazcala, Universidad Autonoma de Queretaro. Queretaro, Mexico.
J Biosci. 2017 Jun;42(2):245-250. doi: 10.1007/s12038-017-9682-9.
Capsinoids are non-pungent analogues of capsaicinoids in pepper (Capsicum spp). The absence of pungency, in addition to their biological activities similar to that of capsaicinoids such as anti-inflammatory, antimicrobial, and antioxidant properties, makes capsinoids an excellent option for increasing use in human and animal nutrition, as well as health and pharmaceutical industries. There are only few sources of pepper producing capsinoids, and one of them (accession 509-45-1), Capsicum annuum L., is a potential source for increasing capsinoids content using strategies as controlled elicitation during plant production in the greenhouse. In this research we evaluated the effect of weekly and one-day-before-harvest foliar applications of hydrogen peroxide, salicylic acid and a xyloglucan oligosaccharide on the concentration of capsiate in fruits of this pepper accession, as well as the gene expression of phenylalanine ammonia-lyase (pal), putative aminotransferase (pamt), capsaicin synthase (at3) and β-keto acyl synthase (kas). Results showed that the two tested concentrations of H2O2 significantly increased capsiate content and gene expression associated with capsaicinoids (pamt, at3 and kas) and the phenylpropanoids (pal) pathways. Plant yield was not affected using this induction strategy. Our results indicated that the pre-harvest and weekly application of hydrogen peroxide and xyloglucan oligosaccharide improved production of capsiate in C. annuum L.
辣椒素类似物是辣椒（辣椒属）中辣椒素的无辣味类似物。除了具有与辣椒素类似的生物活性，如抗炎、抗菌和抗氧化特性外，其无辣味的特点使得辣椒素类似物成为在人类和动物营养以及健康和制药行业中越来越多地被使用的理想选择。只有少数几种辣椒来源能产生辣椒素类似物，其中之一（编号509 - 45 - 1），即辣椒（Capsicum annuum L.），是通过在温室植物生产过程中采用可控诱导等策略来提高辣椒素类似物含量的潜在来源。在本研究中，我们评估了在收获前一周和收获前一天叶面喷施过氧化氢、水杨酸和一种木葡聚糖寡糖对该辣椒品种果实中辣椒素含量以及苯丙氨酸解氨酶（pal）、假定转氨酶（pamt）、辣椒素合成酶（at3）和β - 酮酰基合成酶（kas）基因表达的影响。结果表明，两种测试浓度的过氧化氢均显著提高了与辣椒素（pamt、at3和kas）和苯丙烷类（pal）途径相关的辣椒素含量和基因表达。采用这种诱导策略并未影响植物产量。我们的结果表明，在收获前和每周喷施过氧化氢和木葡聚糖寡糖可提高辣椒（Capsicum annuum L.）中辣椒素的产量。
