Chen Xin, Gao Yang, Li Deheng, Cao Yiqun, Hao Bin
Department of Brain and Spine Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
J Cell Biochem. 2017 Dec;118(12):4897-4904. doi: 10.1002/jcb.26175. Epub 2017 Aug 23.
Gliomas are the most common brain tumors of the center nervous system. And long non-coding RNAs (lncRNAs) are non-protein coding transcripts, which have been considered as one type of gene expression regulator for cancer development. In this study, we investigated the role of lncRNA-TP53TG1 in response to glucose deprivation in human gliomas. The expression levels of TP53TG1 in glioma tissues and cells were analyzed by qRT-PCR. In addition, the influence of TP53TG1 on glucose metabolism related genes at the mRNA level during both high and low glucose treatment was detected by qRT-PCR. MTT, clonogenicity assays, and flow cytometry were performed to detect the cell proliferation and cell apoptosis. Furthermore, the migration of glioma cells was examined by Transwell assays. The expression of TP53TG1 was significantly higher in human glioma tissues or cell lines compared with normal brain tissue or NHA. Moreover, TP53TG1 and some tumor glucose metabolism related genes, such as GRP78, LDHA, and IDH1 were up-regulated significantly in U87 and LN18 cells under glucose deprivation. In addition, knockdown of TP53TG1 decreased cell proliferation and migration and down-regulated GRP78 and IDH1 expression levels and up-regulated PKM2 levels in U87 cells under glucose deprivation. However, over-expression of TP53TG1 showed the opposite tendency. Moreover, the effects of TP53TG1 were more remarkable in low glucose than that in high glucose. Our data showed that TP53TG1 under glucose deprivation may promote cell proliferation and migration by influencing the expression of glucose metabolism related genes in glioma. J. Cell. Biochem. 118: 4897-4904, 2017. © 2017 Wiley Periodicals, Inc.
胶质瘤是中枢神经系统最常见的脑肿瘤。长链非编码RNA（lncRNAs）是一类非蛋白质编码转录本，被认为是癌症发展过程中的一种基因表达调节因子。在本研究中，我们调查了lncRNA-TP53TG1在人类胶质瘤中对葡萄糖剥夺的反应作用。通过qRT-PCR分析了TP53TG1在胶质瘤组织和细胞中的表达水平。此外，通过qRT-PCR检测了在高糖和低糖处理期间，TP53TG1在mRNA水平上对葡萄糖代谢相关基因的影响。进行MTT、克隆形成试验和流式细胞术以检测细胞增殖和细胞凋亡。此外，通过Transwell试验检测胶质瘤细胞的迁移。与正常脑组织或NHA相比，TP53TG1在人类胶质瘤组织或细胞系中的表达明显更高。此外，在葡萄糖剥夺条件下，U87和LN18细胞中TP53TG1以及一些肿瘤葡萄糖代谢相关基因，如GRP78、LDHA和IDH1显著上调。此外，在葡萄糖剥夺条件下，敲低TP53TG1可降低U87细胞的增殖和迁移，并下调GRP78和IDH1的表达水平，上调PKM2水平。然而，TP53TG1的过表达则表现出相反的趋势。此外，TP53TG1在低糖条件下的作用比在高糖条件下更显著。我们的数据表明，在葡萄糖剥夺条件下，TP53TG1可能通过影响胶质瘤中葡萄糖代谢相关基因的表达来促进细胞增殖和迁移。《细胞生物化学杂志》118: 4897 - 4904, 2017。© 2017威利期刊公司
