Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital, Zhengzhou 450003, Henan, China.
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
J Cell Biochem. 2018 Jan;119(1):338-346. doi: 10.1002/jcb.26182. Epub 2017 Jun 27.
The aim of our study was to investigate the effects of miR-133a-3p on human oral squamous cell carcinoma (OSCC) cells by regulating gene COL1A1. OSCC tissues, adjacent tongue epithelial tissues, the immortalized oral epithelial cell line HIOEC, and OSCC cell lines (CAL-27, TCA-8113, SCC-4, SCC-9, and SCC-15) were used in this research. Quantitative real-time PCR (RT-qPCR) was employed to determine the expression of miR-133a-3p and COL1A1. Dual luciferase reporter gene assay and Western blot were applied to verify the binding relationship between miR-133a-3p and COL1A1. Functional assays were also conducted in this study, including CCK-8 assay, colony formation assay, flow cytometry analysis as well as Transwell assay. MiR-133a-3p was found low-expressed both in OSCC tissues and cells lines compared with normal tissues and cell line, respectively, whereas COL1A1 was just the opposite. The over-expression of miR-133a-3p or the down-regulation of COL1A1 suppressed the proliferation, invasion, and mitosis of OSCC cells, whereas simultaneous down-regulation of miR-133a-3p and up-regulation of COL1A1 led to no significant alteration of cell activities. MiR-133a-3p could inhibit the proliferation and migration of OSCC cells through directly targeting COL1A1 and reducing its expression. J. Cell. Biochem. 119: 338-346, 2018. © 2017 Wiley Periodicals, Inc.
本研究旨在通过调控基因 COL1A1 来探究 miR-133a-3p 对人口腔鳞状细胞癌（OSCC）细胞的影响。本研究使用了 OSCC 组织、相邻舌上皮组织、永生化口腔上皮细胞系 HIOEC 和 OSCC 细胞系（CAL-27、TCA-8113、SCC-4、SCC-9 和 SCC-15）。采用实时定量 PCR（RT-qPCR）检测 miR-133a-3p 和 COL1A1 的表达。双荧光素酶报告基因检测和 Western blot 用于验证 miR-133a-3p 和 COL1A1 之间的结合关系。本研究还进行了功能测定，包括 CCK-8 测定、集落形成测定、流式细胞术分析和 Transwell 测定。与正常组织和细胞系相比，OSCC 组织和细胞系中 miR-133a-3p 的表达均较低，而 COL1A1 的表达则相反。过表达 miR-133a-3p 或下调 COL1A1 抑制了 OSCC 细胞的增殖、侵袭和有丝分裂，而同时下调 miR-133a-3p 和上调 COL1A1 则导致细胞活性无明显改变。miR-133a-3p 可通过直接靶向 COL1A1 并降低其表达来抑制 OSCC 细胞的增殖和迁移。J. Cell. Biochem. 119: 338-346, 2018. © 2017 Wiley Periodicals, Inc.
