Wang Kai, Jin Jun, Ma Tengxiao, Zhai Hongfeng
Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan, China.
Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan, China.
Biomed Pharmacother. 2017 Jul;91:517-525. doi: 10.1016/j.biopha.2017.04.050. Epub 2017 May 5.
To test the influence of miR-376c-3p on the proliferation, invasion, migration, cell cycle and apoptosis of human oral squamous cancer cells (OSCC) and the relevant mechanism.
We applied qRT-PCR and Western blot to compare the expression level of miR-376c-3p and HOXB7 in SCC-4, SCC-9, SCC-15, SCC-25 OSCC cell lines and 49 paired OSCC and normal oral epithelial tissue specimens were included in our present study. Also we analyzed the relative relationship of expression level between miR-376c-3p and HOXB7 in cancer tissues. Luciferase assay was used to confirm the target relationship between miR-376c-3p and HOXB7. Besides, MTT, Transwell, wound healing, colony formation and flow cytometer experiments were applied to evaluate the proliferation, cell viability, apoptosis, invasion and migration of transfected OSCC.
MiR-376c-3p was down-regulated while HOXB7 was up-regulated in OSCC tissues and cells than the normal ones. MiR-376c-3p directly targeted HOXB7 and reduced the expression of HOXB7. Overexpression of miR-376c-3p attenuated proliferation of SCC-9, SCC-15, SCC-24 and SCC-25 cells. Moreover, miR-376c-3p suppressed proliferation, viability, migration and invasion and induced G1/G0 arrest and cell apoptosis of SCC-25 cells. Besides, overexpression of HOXB7 efficiently abrogates these influences caused by overexpression of miR-376c-3p.
MiR-376c-3p suppresses the fission, proliferation, migration and invasion and induces cell apoptosis of OSCC via targeting HOXB7.
检测miR-376c-3p对人口腔鳞状癌细胞（OSCC）增殖、侵袭、迁移、细胞周期及凋亡的影响及其相关机制。
应用qRT-PCR和蛋白质免疫印迹法比较miR-376c-3p和HOXB7在SCC-4、SCC-9、SCC-15、SCC-25 OSCC细胞系中的表达水平，本研究纳入49对OSCC及正常口腔上皮组织标本。同时分析癌组织中miR-376c-3p与HOXB7表达水平的相关性。采用荧光素酶报告基因实验验证miR-376c-3p与HOXB7的靶向关系。此外，应用MTT、Transwell、划痕实验、集落形成实验及流式细胞仪实验评估转染后OSCC的增殖、细胞活力、凋亡、侵袭及迁移能力。
与正常组织和细胞相比，OSCC组织和细胞中miR-376c-3p表达下调，HOXB7表达上调。miR-376c-3p直接靶向HOXB7并降低其表达。miR-376c-3p过表达减弱了SCC-9、SCC-15、SCC-24和SCC-25细胞的增殖。此外，miR-376c-3p抑制SCC-25细胞的增殖、活力、迁移和侵袭，并诱导G1/G0期阻滞和细胞凋亡。此外，HOXB7过表达有效消除了miR-376c-3p过表达所引起的这些影响。
miR-376c-3p通过靶向HOXB7抑制OSCC的分裂、增殖、迁移和侵袭并诱导细胞凋亡。
