KAIST Institute for the BioCentury, Department of Biological Sciences, ‡Graduate School of Medical Science and Engineering, and §KAIST Institute for the NanoCentury, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST) , 291 Daehak-ro, Daejeon 34141, Republic of Korea.
ACS Appl Mater Interfaces. 2017 Jun 14;9(23):19736-19745. doi: 10.1021/acsami.7b06899. Epub 2017 Jun 1.
As reports of multidrug resistant pathogens have increased, patients with implanted medical catheters increasingly need alternative solutions to antibiotic treatments. As most catheter-related infections are directly associated with biofilm formation on the catheter surface, which, once formed, is difficult to eliminate, a promising approach to biofilm prevention involves inhibiting the initial adhesion of bacteria to the surface. In this study, we report an amphiphilic, antifouling polymer, poly(DMA-mPEGMA-AA) that can facilely coat the surfaces of commercially available catheter materials in water and prevent bacterial adhesion to and subsequent colonization of the surface, giving rise to an antibiofilm surface. The antifouling coating layer was formed simply by dipping a model substrate (polystyrene, PET, PDMS, or silicon-based urinary catheter) in water containing poly(DMA-mPEGMA-AA), followed by characterization by X-ray photoelectron spectroscopy (XPS). The antibacterial adhesion properties of the polymer-coated surface were assessed for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) growth under static (incubation in the presence of a bacterial suspension) and dynamic (bacteria suspended in a solution under flow) conditions. Regardless of the conditions, the polymer-coated surface displayed significantly reduced attachment of the bacteria (antiadhesion effect > ∼8-fold) compared to the bare noncoated substrates. Treatment of the implanted catheters with S. aureus in vivo further confirmed that the polymer-coated silicon urinary catheters could significantly reduce bacterial adhesion and biofilm formation in a bacterial infection animal model. Furthermore, the polymer-coated catheters did not induce hemolysis and were resistant to the adhesion of blood-circulating cells, indicative of high biocompatibility. Collectively, the present amphiphilic antifouling polymer is potentially useful as a coating platform that renders existing medical devices resistant to biofilm formation.
随着耐药病原体报告的增加，越来越多的植入式医疗导管患者需要替代抗生素治疗的方法。由于大多数导管相关感染直接与导管表面的生物膜形成有关，而一旦形成，就很难消除，因此预防生物膜的一种有前途的方法涉及抑制细菌最初与表面的粘附。在这项研究中，我们报告了一种两亲性、抗污的聚合物，聚（DMA-mPEGMA-AA），它可以在水中轻易地涂覆市售导管材料的表面，并防止细菌粘附和随后在表面的定植，从而产生抗生物膜表面。抗污涂层是通过将模型基底（聚苯乙烯、PET、PDMS 或基于硅的导尿管）简单地浸入含有聚（DMA-mPEGMA-AA）的水中，然后通过 X 射线光电子能谱（XPS）进行表征来形成的。通过静态（在细菌悬浮液存在下孵育）和动态（在溶液中悬浮细菌的情况下）条件下评估了聚合物涂覆表面对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）生长的抗菌粘附性能。无论条件如何，与未涂覆的基底相比，聚合物涂覆表面显示出细菌附着的显著减少（抗粘附效果＞∼8 倍）。体内植入导管治疗金黄色葡萄球菌进一步证实，聚合物涂覆的硅制导尿管可以显著减少细菌感染动物模型中的细菌粘附和生物膜形成。此外，聚合物涂覆的导管不会引起溶血，并且抵抗血液循环细胞的粘附，表明其具有高度的生物相容性。总的来说，这种两亲性抗污聚合物作为涂层平台具有潜在的用途，可使现有医疗器械抵抗生物膜的形成。
