Cohen R M, McLellan C, Dauphin M, Hirata F
Life Sci. 1985 Jan 7;36(1):25-32. doi: 10.1016/0024-3205(85)90282-6.
Treatment of rat cerebral cortical membranes with phospholipase A2 affects, in a parallel fashion, beta-, alpha 1- and alpha 2-adrenergic receptor binding, but not the affinity of these receptors for their respective ligands. Pretreatment of membranes with 0.1 percent glutaraldehyde blocks the effects of phospholipase A2 on adrenergic receptor binding. The results support the hypothesis that desensitization or "masking" of adrenergic receptors may involve changes in membrane lipid composition. Furthermore, glutaraldehyde may prove a useful tool in the investigation of the dynamic roles of lipids in receptor function and more specifically, their regulation and coupling to physiological events.
用磷脂酶A2处理大鼠大脑皮层细胞膜，会以类似的方式影响β-、α1-和α2-肾上腺素能受体结合，但不会影响这些受体对各自配体的亲和力。用0.1%戊二醛预处理细胞膜可阻断磷脂酶A2对肾上腺素能受体结合的影响。这些结果支持这样一种假说，即肾上腺素能受体的脱敏或“掩盖”可能涉及膜脂质组成的变化。此外，戊二醛可能是研究脂质在受体功能中的动态作用，更具体地说，是研究脂质对生理事件的调节及其与之偶联的有用工具。
