Lim Kai-Zheong, Daly Christopher D, Ghosh Peter, Jenkin Graham, Oehme David, Cooper-White Justin, Naidoo Taryn, Goldschlager Tony
Department of Surgery, Monash University.
Department of Surgery, Monash University; Department of Neurosurgery, Monash University; The Ritchie Centre, Hudson Institute of Medical Research;
J Vis Exp. 2017 May 25(123):55753. doi: 10.3791/55753.
Intervertebral disc degeneration is a significant contributor to the development of back pain and the leading cause of disability worldwide. Numerous animal models of intervertebral disc degeneration have been developed. The ideal animal model should closely mimic the human intervertebral disc with regard to morphology, biomechanical properties and the absence of notochordal cells. The sheep lumbar intervertebral disc model fulfils these criteria. We present an ovine model of intervertebral disc degeneration utilizing a drill bit injury through a lateral retroperitoneal approach. The lateral approach significantly reduces the incision and potential morbidity associated with the traditional anterior approach to the ovine spine. Utilization of a drill-bit method of injury affords the ability to produce a consistent and reproducible injury, of precise dimensions, that initiates a consistent degree of intervertebral disc degeneration. The focal nature of the annular and nucleus pulposus defect more closely mimics the clinical condition of focal intervertebral disc herniation. Sheep recover rapidly following this procedure and are typically mobile and eating within the hour. Intervertebral disc degeneration ensues and sheep undergo necropsy and subsequent analysis at periods from eight weeks. We believe that the drill bit injury model of intervertebral disc degeneration offers advantages over more conventional annular injury models.
椎间盘退变是导致背痛的重要因素，也是全球致残的主要原因。目前已建立了多种椎间盘退变的动物模型。理想的动物模型应在形态、生物力学特性以及脊索细胞缺失方面与人类椎间盘高度相似。绵羊腰椎间盘模型符合这些标准。我们展示了一种通过侧腹膜后入路使用钻头损伤建立的绵羊椎间盘退变模型。这种侧方入路显著减少了与传统绵羊脊柱前路手术相关的切口和潜在发病率。使用钻头损伤方法能够产生尺寸精确、一致且可重复的损伤，引发一致程度的椎间盘退变。纤维环和髓核缺损的局灶性更接近局灶性椎间盘突出的临床情况。绵羊在此手术后恢复迅速，通常在一小时内即可活动和进食。随后会发生椎间盘退变，绵羊在八周后的不同时间段接受尸检及后续分析。我们认为，与更传统的纤维环损伤模型相比，椎间盘退变的钻头损伤模型具有优势。
