Bozkurt Baran, Yagmurlu Kaan, Middlebrooks Erik H, Cayci Zuzan, Cevik Orhun M, Karadag Ali, Moen Sean, Tanriover Necmettin, Grande Andrew W
Department of Neurosurgery, University of Minnesota;
Department of Neurosurgery, Barrow Neurological Institute, St. Josephs Hospital and Medical Center.
J Vis Exp. 2017 May 23(123):55681. doi: 10.3791/55681.
The purpose of this study is to show the methodology for the examination of the white matter connections of the supplementary motor area (SMA) complex (pre-SMA and SMA proper) using a combination of fiber dissection techniques on cadaveric specimens and magnetic resonance (MR) tractography. The protocol will also describe the procedure for a white matter dissection of a human brain, diffusion tensor tractography imaging, and three-dimensional documentation. The fiber dissections on human brains and the 3D documentation were performed at the University of Minnesota, Microsurgery and Neuroanatomy Laboratory, Department of Neurosurgery. Five postmortem human brain specimens and two whole heads were prepared in accordance with Klingler's method. Brain hemispheres were dissected step by step from lateral to medial and medial to lateral under an operating microscope, and 3D images were captured at every stage. All dissection results were supported by diffusion tensor imaging. Investigations on the connections in line with Meynert's fiber tract classification, including association fibers (short, superior longitudinal fasciculus I and frontal aslant tracts), projection fibers (corticospinal, claustrocortical, cingulum, and frontostriatal tracts), and commissural fibers (callosal fibers) were also conducted.
本研究的目的是展示一种方法，该方法结合尸体标本上的纤维解剖技术和磁共振（MR）神经纤维束成像，用于检查辅助运动区（SMA）复合体（前SMA和SMA本部）的白质连接。该方案还将描述人脑白质解剖、扩散张量纤维束成像以及三维记录的程序。人脑的纤维解剖和三维记录在明尼苏达大学神经外科系显微外科与神经解剖实验室进行。按照克林格勒方法制备了5个死后人脑标本和2个完整头部。在手术显微镜下，将脑半球从外侧向内侧、再从内侧向外侧逐步解剖，并在每个阶段采集三维图像。所有解剖结果均得到扩散张量成像的支持。还按照迈内特纤维束分类法对连接进行了研究，包括联合纤维（短纤维、上纵束I和额斜束）、投射纤维（皮质脊髓束、屏状核皮质束、扣带束和额纹状体束）以及连合纤维（胼胝体纤维）。
