从珠蛋白信使核糖核酸和胞质溶胶蛋白体外重建信使核糖核蛋白颗粒。
In vitro reconstitution of messenger ribonucleoprotein particles from globin messenger RNA and cytosol proteins.
作者信息
Gaedigk R, Oehler S, Köhler K, Setyono B
出版信息
FEBS Lett. 1985 Jan 7;179(2):201-7. doi: 10.1016/0014-5793(85)80518-4.
Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can be crosslinked to the mRNA are concerned. Proteins thus identified bind specifically to mRNA and not to ribosomal RNA or any synthetic oligonucleotides, with one exception: a 78-kDa protein could be cross-linked to poly(A).
脱蛋白的珠蛋白多聚腺苷酸（poly(A)）+信使核糖核酸（mRNAs）在体外很容易与细胞质中的可溶性RNA结合蛋白重新结合；由此获得的重组信使核糖核蛋白复合物，就硫酸铯（Cs2SO4）中的浮力密度以及可与mRNA交联的蛋白质组成而言，与天然珠蛋白多核糖体-mRNP非常相似。如此鉴定出的蛋白质特异性地结合mRNA，而不结合核糖体RNA或任何合成寡核苷酸，但有一个例外：一种78千道尔顿的蛋白质可与多聚腺苷酸（poly(A)）交联。
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