利用一种可靶向溶酶体且对酸性pH有激活作用的荧光探针，对活细胞和动物中的甲醛进行双光子成像。
Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe.
作者信息
Xie Xilei, Tang Fuyan, Shangguan Xiaoyan, Che Shiyi, Niu Jinye, Xiao Yongsheng, Wang Xu, Tang Bo
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.
出版信息
Chem Commun (Camb). 2017 Jun 13;53(48):6520-6523. doi: 10.1039/c7cc03050a.
Lyso-TPFP presents lysosomal targetability and an acidic pH-activatable response toward formaldehyde. Thus, it exclusively visualizes lysosomal formaldehyde and is immune against it in neutral cytosol and other organelles. In addition, two-photon fluorescence imaging endows Lyso-TPFP with the capability of in situ tracking formaldehyde in live cells and animals.
溶酶体靶向荧光探针Lyso-TPFP对甲醛具有溶酶体靶向性和酸性pH激活响应。因此，它仅能可视化溶酶体中的甲醛，在中性细胞质和其他细胞器中对甲醛免疫。此外，双光子荧光成像赋予Lyso-TPFP在活细胞和动物体内原位追踪甲醛的能力。
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