Ishchenko Andrii, Abola Enrique E, Cherezov Vadim
Department of Chemistry, Bridge Institute, University of Southern California, Los Angeles, CA, 90089, USA.
Methods Mol Biol. 2017;1607:117-141. doi: 10.1007/978-1-4939-7000-1_5.
Membrane proteins are crucial components of cellular membranes and are responsible for a variety of physiological functions. The advent of new tools and technologies for structural biology of membrane proteins has led to a significant increase in the number of structures deposited to the Protein Data Bank during the past decade. This new knowledge has expanded our fundamental understanding of their mechanism of function and contributed to the drug-design efforts. In this chapter we discuss current approaches for membrane protein expression, solubilization, crystallization, and data collection. Additionally, we describe the protein quality-control assays that are often instrumental as a guideline for a shorter path toward the structure.
膜蛋白是细胞膜的关键组成部分，负责多种生理功能。过去十年间，用于膜蛋白结构生物学研究的新工具和新技术不断涌现，使得存入蛋白质数据库的膜蛋白结构数量大幅增加。这些新知识拓展了我们对其功能机制的基本理解，并推动了药物设计工作。在本章中，我们将讨论膜蛋白表达、溶解、结晶及数据收集的当前方法。此外，我们还将介绍蛋白质质量控制检测方法，这些方法通常有助于为更快速地解析结构提供指导。
