膜蛋白结构的原子水平分析。
Atomic-level analysis of membrane-protein structure.
作者信息
Hendrickson Wayne A
机构信息
Department of Biochemistry and Molecular Biophysics and the Department of Physiology and Cellular Biophysics, Columbia University, New York, New York, USA, and at the New York Structural Biology Center (NYSBC), New York, New York, USA.
出版信息
Nat Struct Mol Biol. 2016 Jun 7;23(6):464-7. doi: 10.1038/nsmb.3215.
Abstract
Membrane proteins are substantially more challenging than natively soluble proteins as subjects for structural analysis. Thus, membrane proteins are greatly underrepresented in structural databases. Recently, focused consortium efforts and advances in methodology for protein production, crystallographic analysis and cryo-EM analysis have accelerated the pace of atomic-level structure determination of membrane proteins.
摘要
作为结构分析的对象，膜蛋白比天然可溶蛋白更具挑战性。因此，膜蛋白在结构数据库中的占比极低。近来，专门的联合研究工作以及蛋白质生产、晶体学分析和冷冻电镜分析方法的进步，加快了膜蛋白原子级结构测定的步伐。
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