Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence.
作者信息
Nelson Emily V, Schmidt Kristina M
机构信息
Department of Microbiology and National Emerging Infectious Diseases Laboratories, School of Medicine, Boston University, Boston, MA, USA.
Institute for Novel and Emerging Infectious Diseases, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany.
出版信息
Methods Mol Biol. 2017;1628:211-225. doi: 10.1007/978-1-4939-7116-9_17.
In this chapter, the use of immunofluorescence analysis as a tool to examine stress granule (SG) formation in Ebola virus (EBOV)-infected cells is described. The following protocol focuses on the process of inducing and analyzing the cellular stress response, including treatment of cells with inducers and inhibitors of the SG formation, and also describes EBOV infection, DNA transfection, and the usage of different cell lines.
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