Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells.

作者信息

Olejnik Judith, Nelson Emily V

机构信息

Department of Microbiology and National Emerging Infectious Diseases Laboratories, School of Medicine, Boston University, Boston, MA, USA.

出版信息

Methods Mol Biol. 2017;1628:227-241. doi: 10.1007/978-1-4939-7116-9_18.

DOI:10.1007/978-1-4939-7116-9_18

PMID:28573624

Abstract

In this chapter, we describe a Western blot assay for the successful detection of apoptosis in Ebola virus (EBOV)-infected cells. The protocol includes all steps from cell culture, infection of cells, generation of lysates, and analysis using Western blot to detect caspase cleavage as marker of apoptosis.

摘要