Mibei Elias K, Owino Willis O, Ambuko Jane, Giovannoni James J, Onyango Arnold N
Department of Food Science and Technology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
Department of Plant Science and Crop Protection, University of Nairobi, Nairobi, Kenya.
J Sci Food Agric. 2018 Jan;98(1):205-216. doi: 10.1002/jsfa.8458. Epub 2017 Aug 3.
Drought stress is one of the main abiotic stresses that affect crops. It leads to biochemical changes that can have adverse effects on plant growth, development and productivity. African eggplants are important vegetable and fruit crops reported to adapt and thrive well under drought stress. The diversified metabolites arising due to stress have not been well defined. A gas chromatographic-mass spectrometric metabolomic approach was applied to characterize the effect of drought stress on metabolites at different stages of growth. Nineteen accessions were selected for analysis and drought was imposed by withholding water until soil moisture reached 60% field capacity. Fresh leaf tissues were sampled before stress, 2 and 4 weeks after stress and metabolite profiling done.
Significant changes in metabolite content were observed, and potentially important metabolites with respect to stress responses were characterized. Proline, glutamate, sucrose, fructose and tricarboxylic acid cycle metabolites were shown to be positively correlated with stress. Principal component analysis showed a clear discrimination between the different accessions, growth stages and stress/control conditions.
The results illustrate that drought stress has a significant impact on the concentrations of some metabolites, such as amino acids, sugars and organic acids, which may contribute to drought stress effects and tolerance. © 2017 Society of Chemical Industry.
干旱胁迫是影响作物的主要非生物胁迫之一。它会导致生化变化，对植物生长、发育和生产力产生不利影响。非洲茄子是重要的蔬菜和水果作物，据报道能在干旱胁迫下适应并良好生长。因胁迫产生的多种代谢产物尚未得到很好的界定。采用气相色谱 - 质谱代谢组学方法来表征干旱胁迫对不同生长阶段代谢产物的影响。选择了19个种质进行分析，通过 withholding water 直至土壤湿度达到田间持水量的60%来施加干旱胁迫。在胁迫前、胁迫后2周和4周采集新鲜叶片组织样本并进行代谢产物分析。
观察到代谢产物含量有显著变化，并鉴定出了与胁迫反应潜在相关的重要代谢产物。脯氨酸、谷氨酸、蔗糖、果糖和三羧酸循环代谢产物与胁迫呈正相关。主成分分析显示不同种质、生长阶段以及胁迫/对照条件之间有明显区分。
结果表明干旱胁迫对某些代谢产物的浓度有显著影响，如氨基酸类、糖类和有机酸类，这可能导致干旱胁迫效应和耐受性。© 2017 化学工业协会 。
原文中“withholding water”直译为“扣留水分”，结合语境这里可能是指“控制水分供应”之类的意思，但按要求不添加解释，保留原文表述。
