Guo Xiaoyang, Xin Zeyu, Yang Tiegang, Ma Xingli, Zhang Yang, Wang Zhiqiang, Ren Yongzhe, Lin Tongbao
College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Plants (Basel). 2020 Apr 17;9(4):520. doi: 10.3390/plants9040520.
Metabolomics is an effective biotechnological tool that can be used to attain comprehensive information on metabolites. In this study, the profiles of metabolites produced by wheat seedlings in response to drought stress were investigated using an untargeted approach with ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to determine various physiological processes related to drought tolerance from the cross between drought-tolerant genotype (HX10) and drought-sensitive genotype (YN211). The current study results showed that under drought stress, HX10 exhibited higher growth indices than YN211. After drought stress treatment, a series of phenolics accumulated higher in HX10 than in YN211, whereas the amount of thymine, a pyrimidine, is almost 13 folds of that in YN211. These metabolites, as well as high levels of different amino acids, alkaloids, organic acids, and flavonoids in the drought treated HX10 could help to explain its strong drought-tolerant capacity. The current study explored the understanding of the mechanisms involved in the drought response of wheat seedling; these metabolome data could also be used for potential QTL or GWAS studies to identify locus (loci) or gene(s) associated with these metabolic traits for the crop improvement.
代谢组学是一种有效的生物技术工具，可用于获取有关代谢物的全面信息。在本研究中，采用超高效液相色谱-质谱联用（UPLC-MS）的非靶向方法，研究了耐旱基因型（HX10）和干旱敏感基因型（YN211）杂交后代小麦幼苗在干旱胁迫下产生的代谢物谱，以确定与耐旱性相关的各种生理过程。当前研究结果表明，在干旱胁迫下，HX10的生长指标高于YN211。干旱胁迫处理后，一系列酚类物质在HX10中的积累量高于YN211，而嘧啶胸腺嘧啶的含量几乎是YN211的13倍。这些代谢物，以及干旱处理后的HX10中高水平的不同氨基酸、生物碱、有机酸和黄酮类化合物，有助于解释其强大的耐旱能力。当前研究探索了对小麦幼苗干旱响应机制的理解；这些代谢组数据也可用于潜在的QTL或GWAS研究，以鉴定与这些代谢性状相关的位点或基因，用于作物改良。
