Halder Madhumanti, Mookerjee Subham, Batabyal Prasenjit, Palit Anup
a Division of Bacteriology , National Institute of Cholera & Enteric Diseases, (Indian Council of Medical Research) , Kolkata , India.
Int J Environ Health Res. 2017 Aug;27(4):241-251. doi: 10.1080/09603123.2017.1332346. Epub 2017 Jun 2.
Diarrhoea still remains an unsolved enigma in developing countries, a major concern for the health planners. We targeted the abundance and toxicity of Vibrio cholerae non-O1/non-O139 (NOVC) in Gangetic riverine-estuarine ecosystem. A total of 74 V. cholerae were isolated from 120 water samples (68 NOVC, 6 V. cholerae O1) from two sampling sites off river Ganges. V. cholerae showed distinct seasonality, with steady increase from summer to monsoon, steep ascent in post-monsoon and an abrupt decline in winter. Highest number of NOVC was isolated form Howrah, attributed to low salinity and high anthropogenic influence. Environmental NOVC harboured hlyA (94.0 %), rtxA (81.0 %) and toxR (28.0 %) genes. About 23.4 % of the hlyA harbouring NOVC showed haemolytic activity. Accessory toxin genes (tlcR, toxT, RJ and LJ and aldA), among 3-5 % of the NOVC carry significant health implications. Haemolytic activity and biofilm formation in NOVC, during unfavourable conditions, facilitates gene transfer and emphasises the role of environmental NOVC in diarrhoeal incidence in South Bengal, India.
腹泻在发展中国家仍然是一个未解之谜，也是卫生规划者主要关注的问题。我们针对恒河河口生态系统中霍乱弧菌非O1/非O139（NOVC）的丰度和毒性进行了研究。从恒河两个采样点的120份水样（68份NOVC，6份霍乱弧菌O1）中总共分离出74株霍乱弧菌。霍乱弧菌表现出明显的季节性，从夏季到季风季节稳步增加，季风后急剧上升，冬季则突然下降。从豪拉分离出的NOVC数量最多，这归因于低盐度和高人为影响。环境中的NOVC携带hlyA（94.0%）、rtxA（81.0%）和toxR（28.0%）基因。约23.4%携带hlyA的NOVC表现出血清溶解活性。3%-5%的NOVC中存在的辅助毒素基因（tlcR、toxT、RJ和LJ以及aldA）具有重大的健康影响。在不利条件下，NOVC中的溶血活性和生物膜形成促进了基因转移，并强调了环境中的NOVC在印度西孟加拉邦腹泻发病率中的作用。
