Al-Sa'ady A T, Baqer K A, Al-Salim Z K S
Department of Clinical Laboratory Sciences, Faculty of Pharmacy, University of Babylon, Hillah, Iraq.
Department of Microbiology, Al Imam Al Sadiq Teaching Hospital, Hillah, Iraq.
New Microbes New Infect. 2020 Aug 7;37:100739. doi: 10.1016/j.nmni.2020.100739. eCollection 2020 Sep.
is a cause of serious endemic diarrhoea associated with cholera in many regions in the world. A total of 256 stool and rectal swabs were collected from patients suspected to have cholera admitted to three hospitals in Hillah, Babylon Governorate, Iraq, for the period 1 September to 29 December 2017. After the routine culture of samples for isolation and identification of isolates, PCR was performed for molecular detection of isolates based on 16S ribosomal RNA gene. Toxigenicity was detected by RTX toxin genes. PCR technique emphasized molecular detection of for eight isolates. Only two isolates (25%) possessed both the and genes, while only three isolates (37.5%) possessed the gene. DNA sequencing was performed for the eight isolates via analysis and phylogenetic tree. The observed bacterial variants were compared to their neighbour homologous reference sequences using the National Center for Biotechnology Information (NCBI) BLAST server (Basic Local Alignment Search Tool; https://blast.ncbi.nlm.nih.gov/Blast.cgi). The findings indicated that the eight investigated isolates of were positioned in three different phylogenetic positions. Partial sequence dissimilarities were reported between GenBank isolate accession number MK212155.1 and these six clustered GenBank accession numbers of the same species. For the first time in Babylon Governorate, Iraq, the molecular assay, sequencing and phylogenetic tree are reported for and their toxins isolated during the 2017 cholera outbreak.
是世界上许多地区与霍乱相关的严重地方性腹泻的一个病因。在2017年9月1日至12月29日期间，从伊拉克巴比伦省希拉市三家医院收治的疑似霍乱患者中总共采集了256份粪便和直肠拭子。在对样本进行常规培养以分离和鉴定菌株后，基于16S核糖体RNA基因对菌株进行PCR分子检测。通过RTX毒素基因检测产毒性。PCR技术着重对8株菌株进行分子检测。只有两株菌株（25%）同时拥有 和 基因，而只有三株菌株（37.5%）拥有 基因。通过分析和系统发育树对这8株菌株进行DNA测序。使用美国国立生物技术信息中心（NCBI）的BLAST服务器（基本局部比对搜索工具；https://blast.ncbi.nlm.nih.gov/Blast.cgi）将观察到的细菌变体与其邻近的同源参考序列进行比较。研究结果表明，所研究的8株 菌株位于三个不同的系统发育位置。报告了GenBank分离株登录号MK212155.1与同一物种的这六个聚类GenBank登录号之间的部分序列差异。在伊拉克巴比伦省，首次报告了2017年霍乱疫情期间分离出的 及其毒素的分子检测、测序和系统发育树。
