Berman Sean, Uhlendorf Toni L, Mills David K, Lander Elliot B, Berman Mark H, Cohen Randy W
School of Biological Sciences, Louisiana Tech University, Ruston, LA 71272, USA.
Department of Biology, California State University Northridge, Northridge, CA 91330, USA.
Brain Sci. 2017 Jun 2;7(6):59. doi: 10.3390/brainsci7060059.
This study looked to validate the acoustic wave technology of the Storz-D-Actor that inflicted a consistent closed-head, traumatic brain injury (TBI) in rats. We studied a range of single pulse pressures administered to the rats and observed the resulting decline in motor skills and memory. Histology was observed to measure and confirm the injury insult.
Four different acoustic wave pressures were studied using a single pulse: 0, 3.4, 4.2 and 5.0 bar ( = 10 rats per treatment group). The pulse was administered to the left frontal cortex. Rotarod tests were used to monitor the rats' motor skills while the water maze test was used to monitor memory deficits. The rats were then sacrificed ten days post-treatment for histological analysis of TBI infarct size.
The behavioral tests showed that acoustic wave technology administered an effective insult causing significant decreases in motor abilities and memory. Histology showed dose-dependent damage to the cortex infarct areas only.
This study illustrates that the Storz D-Actor effectively induces a repeatable TBI infarct, avoiding the invasive procedure of a craniotomy often used in TBI research.
本研究旨在验证施托兹D - 演员型（Storz-D-Actor）的声波技术，该技术可在大鼠身上造成一致的闭合性颅脑创伤性脑损伤（TBI）。我们研究了施加于大鼠的一系列单脉冲压力，并观察由此导致的运动技能和记忆力下降情况。通过组织学观察来测量和确认损伤情况。
使用单脉冲研究了四种不同的声波压力：0、3.4、4.2和5.0巴（每个治疗组10只大鼠）。脉冲施加于左额叶皮质。使用转棒试验监测大鼠的运动技能，同时使用水迷宫试验监测记忆缺陷。然后在治疗后10天处死大鼠，对TBI梗死灶大小进行组织学分析。
行为测试表明，声波技术造成了有效的损伤，导致运动能力和记忆力显著下降。组织学显示仅皮质梗死区域存在剂量依赖性损伤。
本研究表明，施托兹D - 演员型能有效诱导可重复的TBI梗死灶，避免了TBI研究中常用的开颅侵入性操作。
