Yang Li, You Jun, Wang Yanping, Li Jinzhu, Quan Wenli, Yin Mingzhu, Wang Qingfeng, Chan Zhulong
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, Hubei, 430074, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei, 430062, China.
Plant Physiol Biochem. 2017 Aug;117:1-11. doi: 10.1016/j.plaphy.2017.05.013. Epub 2017 May 25.
The 14-3-3 proteins are highly conserved and ubiquitously found in eukaryotes. Plant 14-3-3 proteins are involved in many signaling pathways to regulate plant growth and development. Here we identified seven Brachypodium distachyon 14-3-3 genes and analyzed the evolution, gene structure and expression profiles of these genes. Several cis-elements involved in stress response and hormone pathway were found in the promoter region of 14-3-3 genes. Results of gene expression analysis showed that these genes were induced by abiotic stresses or hormone treatments. Transgenic Arabidopsis overexpressing BdGF14a exhibited increased leaf water content (LWC) and decreased electrolyte leakage (EL) and showed improved drought stress tolerance. BdGF14a transgene significantly up-regulated expression levels of DREB1A and DREB1B, but slightly elevated ABI1 expression. These results indicated that BdGF14a functioned as a positive regulator in plant response to drought stress mainly via ABA independent pathway.
14-3-3蛋白高度保守，广泛存在于真核生物中。植物14-3-3蛋白参与许多信号通路，以调节植物的生长和发育。在此，我们鉴定了7个二穗短柄草14-3-3基因，并分析了这些基因的进化、基因结构和表达谱。在14-3-3基因的启动子区域发现了几个参与应激反应和激素途径的顺式元件。基因表达分析结果表明，这些基因受非生物胁迫或激素处理诱导。过表达BdGF14a的转基因拟南芥叶片含水量（LWC）增加，电解质渗漏（EL）减少，干旱胁迫耐受性提高。BdGF14a转基因显著上调DREB1A和DREB1B的表达水平，但略微提高ABI1的表达。这些结果表明，BdGF14a主要通过不依赖ABA的途径在植物对干旱胁迫的响应中起正向调节作用。
