LeRoith D, Pickens W, Crosby L K, Berelowitz M, Holtgrefe M, Shiloach J
Biochim Biophys Acta. 1985 Mar 8;838(3):335-42. doi: 10.1016/0304-4165(85)90231-4.
Extracts of Escherichia coli grown in defined medium contain somatostatin-related material (1-10 pg/g wet weight of cells). Preconditioned medium had no immunoactive somatostatin whereas, conditioned medium had 110-150 pg/l. Following purification of the extracted material on Sep-pak C18, Bio-Gel P-6 and HPLC, multiple molecular weight forms of somatostatin- (SRIF-) related material were identified. The material in one peak reacted in both the N-terminal and C-terminal SRIF immunoassay and coeluted on HPLC with SRIF-28, whereas that in a second peak eluted near SRIF-14 and was reactive only in the C-terminal SRIF assay. The two peaks are thus similar to SRIF-28 and SRIF-14 of vertebrates. These findings add support to the suggestion that vertebrate-type peptide hormones and neuropeptides have early evolutionary origins.
在限定培养基中生长的大肠杆菌提取物含有与生长抑素相关的物质（每克细胞湿重含1 - 10皮克）。预处理培养基中没有免疫活性生长抑素，而条件培养基中有110 - 150皮克/升。在Sep - pak C18、Bio - Gel P - 6和高效液相色谱（HPLC）上对提取物进行纯化后，鉴定出了多种分子量形式的与生长抑素（SRIF）相关的物质。一个峰中的物质在N端和C端SRIF免疫测定中均有反应，并且在HPLC上与SRIF - 28共洗脱，而第二个峰中的物质在SRIF - 14附近洗脱，且仅在C端SRIF测定中有反应。因此，这两个峰类似于脊椎动物的SRIF - 28和SRIF - 14。这些发现支持了脊椎动物型肽激素和神经肽具有早期进化起源这一观点。
