LeRoith D, Pickens W, Vinik A I, Shiloach J
Biochem Biophys Res Commun. 1985 Mar 29;127(3):713-9. doi: 10.1016/s0006-291x(85)80001-2.
Extracts of B. subtilis contain somatostatin-like immunoactivity (1-20 pg per g wet weight cells). Two major forms were detected, one with reactivity in both N- and C-terminal immunoassays similar to somatostatin-28 and a second form reactive only in the C-terminal specific immunoassay similar to somatostatin-14. Both forms were active in a bioassay and the bioactivity was neutralized in the presence of antibody to the central, biologically active part of somatostatin-14. Preconditioned medium contained no detectable somatostatin whereas conditioned medium had 80-380 pg per liter.
枯草芽孢杆菌提取物含有类生长抑素免疫活性（每克湿重细胞含1 - 20皮克）。检测到两种主要形式，一种在N端和C端免疫测定中均有反应，类似于生长抑素 - 28；另一种仅在C端特异性免疫测定中有反应，类似于生长抑素 - 14。两种形式在生物测定中均有活性，且在存在针对生长抑素 - 14中央生物活性部分的抗体时，生物活性被中和。预处理培养基中未检测到可检测的生长抑素，而条件培养基中每升含有80 - 380皮克。
