Wang Chen, Wang Rui, Xu Yuan, Zhang Man, Yang Fan, Sun Shudong, Zhao Changsheng
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:1035-1045. doi: 10.1016/j.msec.2017.04.123. Epub 2017 Apr 26.
In this study, new kinds of heparin-mimicking polyurethanes (PUs) were fabricated conveniently in the mixed solution of dimethyl sulfoxide (DMSO) and water, reducing the usage of organic solvent. Functional groups of SOH and/or COOH were introduced into PUs with various ratios of SOH to COOH. The PUs were then blended with polyethersulfone (PES) to fabricate heparin-mimicking modified PES membranes by a phase inversion technique. Then, the microstructures, zeta potentials, water contact angles (WCA) and protein adsorptions of the membranes were characterized. Comparing with pristine PES membrane, the modified membranes showed changed cross-sectional morphology, lowered zeta potentials and decreased water contact angles, revealing that the hydrophilicity and anti-fouling properties were improved. The modified membranes also showed prolonged clotting tines (APTTs) and suppressed platelet adhesion, revealing that the anticoagulant properties increased. The results of complement activation, contact activation and platelet activation further implied that the modified membranes had good blood compatibility. In addition, the cells on the membranes showed good morphology with the introduction of the PUs. With the increase of the ratio of SOH to COOH, the hydrophilicity, the blood compatibility as well as the cytocompatibility increased, implying that the SOH groups could improve the hemocompatibility of the membranes more effectively than COOH groups. While the membranes containing more COOH groups had better antifouling properties of BSA and BFG. Therefore, the hemocompatibility for the heparin-mimicking membranes could be tuned by controlling the ratios of SOH to COOH. The PES/PU composite membranes might have great potential to be used in the field of blood purification.
在本研究中，新型肝素模拟聚氨酯（PU）在二甲基亚砜（DMSO）和水的混合溶液中方便地制备而成，减少了有机溶剂的使用量。将SOH和/或COOH官能团以不同的SOH与COOH比例引入到PU中。然后，通过相转化技术将PU与聚醚砜（PES）共混以制备肝素模拟改性PES膜。接着，对膜的微观结构、zeta电位、水接触角（WCA）和蛋白质吸附进行了表征。与原始PES膜相比，改性膜的横截面形态发生了变化，zeta电位降低，水接触角减小，表明亲水性和抗污染性能得到了改善。改性膜还表现出凝血时间（APTT）延长和血小板粘附受到抑制，表明抗凝性能增强。补体激活、接触激活和血小板激活的结果进一步表明改性膜具有良好的血液相容性。此外，随着PU的引入，膜上的细胞呈现出良好的形态。随着SOH与COOH比例的增加，亲水性、血液相容性以及细胞相容性均增加，这意味着SOH基团比COOH基团能更有效地改善膜的血液相容性。而含有更多COOH基团的膜对牛血清白蛋白（BSA）和纤维蛋白原（BFG）具有更好的抗污染性能。因此，通过控制SOH与COOH的比例可以调节肝素模拟膜的血液相容性。PES/PU复合膜在血液净化领域可能具有巨大的应用潜力。
