Orunoğlu Merdan, Kaffashi Abbas, Pehlivan Sibel Bozdağ, Şahin Selma, Söylemezoğlu Figen, Oğuz Kader Karli, Mut Melike
Bayburt State Hospital, Department of Neurosurgery, Bayburt, Turkey.
Hacettepe University, Department of Nanotechnology and Nanomedicine, Ankara, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:32-38. doi: 10.1016/j.msec.2017.03.292. Epub 2017 Apr 6.
Curcumin, the active ingredient of turmeric, has a remarkable antitumor activity against various cancers, including glioblastoma. However, it has poor absorption and low bioavailability; thus, to cross the blood-brain barrier and reach tumor tissue, it needs to be transferred to tumor site by special drug delivery systems, such as nanoparticles.
We aimed to evaluate the antitumor activity of curcumin on glioblastoma tissue in the rat glioma-2 (RG2) tumor model when it is loaded on poly(lactic-co-glycolic acid)-1,2-distearoyl-glycerol-3-phospho-ethanolamine-N-[methoxy (polyethylene glycol)-2000] ammonium salt (PLGA-DSPE-PEG) hybrid nanoparticles.
Glioblastoma was induced in 42 adult female Wistar rats (250-300g) by RG2 tumor model. The curcumin-loaded nanoparticles were injected by intravenous (n=6) or intratumoral route (n=6). There were five control groups, each containing six rats. First control group was not applied any treatment. The remaining four control groups were given empty nanoparticles or curcumin alone by intravenous or intratumoral route, respectively. The change in tumor volume was assessed by magnetic resonance imaging and histopathology before and 5days after drug injections.
Tumor size decreased significantly after 5days of intratumoral injection of curcumin-loaded nanoparticle (from 66.6±44.6 to 34.9±21.7mm, p=0.028), whereas it significantly increased in nontreated control group (from 33.9±21.3 to 123.7±41.1mm, p=0.036) and did not significantly change in other groups (p>0.05 for all).
In this in vivo experimental model, intratumoral administration of curcumin-loaded PLGA-DSPE-PEG hybrid nanoparticles was effective against glioblastoma. Curcumine-loaded nanoparticles may have potential application in chemotherapy of glioblastoma.
姜黄素是姜黄的活性成分，对包括胶质母细胞瘤在内的多种癌症具有显著的抗肿瘤活性。然而，其吸收性差且生物利用度低；因此，为了穿过血脑屏障并到达肿瘤组织，需要通过特殊的药物递送系统，如纳米颗粒，将其转运至肿瘤部位。
我们旨在评估负载于聚乳酸-乙醇酸共聚物-1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-[甲氧基（聚乙二醇）-2000]铵盐（PLGA-DSPE-PEG）混合纳米颗粒上的姜黄素对大鼠胶质瘤-2（RG2）肿瘤模型中胶质母细胞瘤组织的抗肿瘤活性。
通过RG2肿瘤模型在42只成年雌性Wistar大鼠（250 - 300g）中诱导胶质母细胞瘤。负载姜黄素的纳米颗粒通过静脉注射（n = 6）或瘤内注射（n = 6）。有五个对照组，每组包含六只大鼠。第一对照组不进行任何治疗。其余四个对照组分别通过静脉或瘤内途径给予空纳米颗粒或单独的姜黄素。在注射药物前和注射后5天通过磁共振成像和组织病理学评估肿瘤体积的变化。
瘤内注射负载姜黄素的纳米颗粒5天后肿瘤大小显著减小（从66.6±44.6减小至34.9±21.7mm，p = 0.028），而未治疗的对照组肿瘤大小显著增加（从33.9±21.3增加至123.7±41.1mm，p = 0.036），其他组无显著变化（所有p>0.05）。
在这个体内实验模型中，瘤内给予负载姜黄素的PLGA-DSPE-PEG混合纳米颗粒对胶质母细胞瘤有效。负载姜黄素的纳米颗粒可能在胶质母细胞瘤化疗中有潜在应用。
