Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Carbohydr Polym. 2017 Sep 1;171:281-291. doi: 10.1016/j.carbpol.2017.05.027. Epub 2017 May 10.
Biocompatible and biodegradable polyurethanes (PUs) based on polycaprolactone diol (PCL) were prepared and filled with cellulose nanowhiskers (CNWs) obtained from wastepaper. The incorporated polyurethane nanocomposites were used to prepare foamed scaffolds with bimodal cell sizes through solvent casting/particulate leaching method. Sodium chloride and sugar porogens were also prepared to fabricate the scaffolds. The mechanical and thermal properties of PU/CNW nanocomposites were investigated. Incorporation of different CNWs resulted in various structures with tunable mechanical properties and biodegradability. All bimodal foam nanocomposites were biodegradable and also non-cytotoxic as revealed by MTT assay using SNL fibroblast cell line. PU/CNW foam scaffolds were used for osteogenic differentiation of human mesenchymal stem cells (hMSCs). Based on the results, such PU/CNW nanocomposites could support proliferation and osteogenic differentiation of hMSCs in three-dimensional synthetic extracellular matrix (ECM).
基于聚己内酯二醇（PCL）的生物相容性和可生物降解型聚氨基甲酸乙酯（PU）被制备出来，并填充了来自废纸的纤维素纳米纤维（CNWs）。所加入的聚氨酯纳米复合材料通过溶剂浇铸/颗粒浸出法被用于制备具有双峰细胞大小的泡沫支架。氯化钠和糖致孔剂也被制备来制造支架。对 PU/CNW 纳米复合材料的机械和热性能进行了研究。不同的 CNWs 的加入导致了具有可调机械性能和可生物降解性的各种结构。所有双峰泡沫纳米复合材料都是可生物降解的，并且通过使用 SNL 成纤维细胞系的 MTT 测定法也显示出非细胞毒性。PU/CNW 泡沫支架被用于人骨髓间充质干细胞（hMSCs）的成骨分化。根据这些结果，这种 PU/CNW 纳米复合材料可以在三维合成细胞外基质（ECM）中支持 hMSCs 的增殖和成骨分化。
