适用于 hMSCs 成骨分化的废纸高纵横比磷酸化钙化冰糖状纤维素纳米晶须。
High aspect ratio phospho-calcified rock candy-like cellulose nanowhiskers of wastepaper applicable in osteogenic differentiation of hMSCs.
机构信息
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
出版信息
Carbohydr Polym. 2017 Nov 1;175:293-302. doi: 10.1016/j.carbpol.2017.08.001. Epub 2017 Aug 2.
The aim of this study was to prepare cellulose nanowhiskers (CNWs) from wastepaper powder (WPP), as an environmentally friendly approach for obtaining the source material, which is a highly available and low-cost precursor for cellulose nanomaterial processing. Acid hydrolysis and calcification treatments were employed for extraction of CNWs and preparation of novel phospho-calcified cellulose nanowhiskers (PCCNWs). CNWs and PCCNWs were analyzed through optical microscopy (OM), scanning electron microscopy (SEM), Fourier-transformed infrared spectra (FTIR) and X-ray diffraction analysis (XRD). Cell behaviors in the presence of CNWs and PCCNWs were studied by MTT assay and live-dead staining. Finally, the effect of these particles on osteogenic differentiation of stem cells was evaluated based on alkaline phosphatase activity (ALP), calcium mineralization as well as von Kossa and alizarin red staining. Based on the results, PCCNWs had a positive effect on osteogenic differentiation of human mesenchymal stem cells (hMSCs) and can be used for developing new approaches for bone tissue engineering.
本研究旨在从废纸粉末（WPP）中制备纤维素纳米纤维（CNWs），这是一种环保的方法，可以获得来源材料，这是一种高度可用且低成本的纤维素纳米材料加工前体。采用酸水解和钙化处理从 CNWs 中提取并制备新型磷酸钙化纤维素纳米纤维（PCCNWs）。通过光学显微镜（OM）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线衍射分析（XRD）对 CNWs 和 PCCNWs 进行分析。通过 MTT 检测和死活染色研究了 CNWs 和 PCCNWs 存在下细胞的行为。最后，基于碱性磷酸酶活性（ALP）、钙矿化以及 von Kossa 和茜素红染色，评估了这些颗粒对干细胞成骨分化的影响。结果表明，PCCNWs 对人骨髓间充质干细胞（hMSCs）的成骨分化有积极影响，可用于开发新的骨组织工程方法。
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