Jafari Hafez, Shahrousvand Mohsen, Kaffashi Babak
School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11365-4563, Enghelab Avenue, Tehran, 1417613131, Iran.
Caspian Faculty of Engineering, College of Engineering, University of Tehran, P.O. Box 119-43841, Chooka Branch, Rezvanshahr, 4386156387, Guilan Province, Iran.
ACS Biomater Sci Eng. 2018 Jul 9;4(7):2484-2493. doi: 10.1021/acsbiomaterials.7b01020. Epub 2018 Jun 4.
In this work, phospho-calcified cellulose nanowhiskers (PCCNWs) were prepared from wastepaper powder (WPP) and were dispersed in poly(ε-caprolactone) (PCL). The biocompatible and biodegradable (PCL)/PCCNW bimodal foam nanocomposites with two species cell sizes were prepared by the solvent casting/particulate leaching method in different weight percentage of PCCNWs. The mechanical, thermal, and in vitro biological properties of PCL/PCCNW nanocomposites were investigated. All PCL/PCCNW scaffolds were hydrophilic, biodegradable, and also noncytotoxic. The human mesenchymal stem cells were cultured on the prepared PCL/PCCNW bimodal foam nanocomposites and differentiated to osteoblasts. On the basis of evaluating tests such as MTT assay, acridine orange/ethidium bromide staining, alkaline phosphatase assay, calcium content assay, and alizarin red staining, PCL/PCCNW scaffolds were introduced as an appropriate option for emulating the behavior of extracellular matrix. Increasing PCCNWs improves the mechanical, hydrophilic, and biodegradability properties of the nanocomposites as well as their osteoconductivity.
在本研究中，由废纸粉末（WPP）制备了磷酸化纤维素纳米晶须（PCCNWs），并将其分散于聚（ε-己内酯）（PCL）中。通过溶剂浇铸/颗粒沥滤法，在不同重量百分比的PCCNWs条件下，制备了具有两种细胞尺寸的生物相容性和可生物降解的（PCL）/PCCNW双峰泡沫纳米复合材料。研究了PCL/PCCNW纳米复合材料的力学、热学和体外生物学性能。所有PCL/PCCNW支架均具有亲水性、可生物降解性且无细胞毒性。将人间充质干细胞培养在制备的PCL/PCCNW双峰泡沫纳米复合材料上，并使其分化为成骨细胞。基于MTT法、吖啶橙/溴化乙锭染色、碱性磷酸酶测定、钙含量测定和茜素红染色等评估测试，PCL/PCCNW支架被认为是模拟细胞外基质行为的合适选择。增加PCCNWs可改善纳米复合材料的力学、亲水性、生物降解性及其骨传导性。
