Unit of Biochemistry, Department of Biology, University of Fribourg, Chemin du Musée 10, CH-1700 Fribourg, Switzerland.
Biochemistry Center Heidelberg (BZH), University of Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Trends Biochem Sci. 2017 Aug;42(8):640-654. doi: 10.1016/j.tibs.2017.05.005. Epub 2017 Jun 1.
The biogenesis of eukaryotic ribosomes is a complicated process during which the transcription, modification, folding, and processing of the rRNA is coupled with the ordered assembly of ∼80 ribosomal proteins (r-proteins). Ribosome synthesis is catalyzed and coordinated by more than 200 biogenesis factors as the preribosomal subunits acquire maturity on their path from the nucleolus to the cytoplasm. Several biogenesis factors also interconnect the progression of ribosome assembly with quality control of important domains, ensuring that only functional subunits engage in translation. With the recent visualization of several assembly intermediates by cryoelectron microscopy (cryo-EM), a structural view of ribosome assembly begins to emerge. In this review we integrate these first structural insights into an updated overview of the consecutive ribosome assembly steps.
真核核糖体的生物发生是一个复杂的过程，在此过程中，rRNA 的转录、修饰、折叠和加工与大约 80 种核糖体蛋白（r 蛋白）的有序组装相偶联。核糖体的合成由 200 多种生物发生因子催化和协调，因为前核糖体亚基在从核仁到细胞质的成熟过程中获得成熟。一些生物发生因子还将核糖体组装的进展与重要结构域的质量控制联系起来，以确保只有功能亚基参与翻译。随着最近通过冷冻电子显微镜（cryo-EM）对几个组装中间体的可视化，核糖体组装的结构视图开始显现。在这篇综述中，我们将这些最初的结构见解整合到核糖体组装步骤的最新概述中。
